The University of British Columbia, Michael Smith Laboratories, 301-2185 East Mall Road, Vancouver, BC, V6T 1Z4, Canada.
Future Microbiol. 2010 Feb;5(2):253-65. doi: 10.2217/fmb.09.114.
Proteomic technology offers an unprecedented systematic approach to investigate the protein complement of any organism. The field of microbial pathogenesis has greatly benefited from other systems approaches, and the application of proteomics to the study of infectious agents is beginning to emerge. Such applications include unambiguously identifying complete virulence factor inventories, studying the response of both host and pathogen to the infection process and elucidating mechanistic actions of virulence factors as they interface with host cells. This review will highlight examples where proteomic studies have contributed to our understanding of pathogenesis in these areas, with an emphasis on pathogens that employ type III and type IV secretion systems. In addition, we will discuss areas where proteomics may help shape further investigation and discovery in this field.
蛋白质组学技术为研究任何生物体的蛋白质组成提供了一种前所未有的系统方法。微生物发病机制领域极大地受益于其他系统方法，蛋白质组学在传染病原体研究中的应用也开始出现。此类应用包括明确鉴定完整的毒力因子目录、研究宿主和病原体对感染过程的反应以及阐明毒力因子与宿主细胞相互作用时的作用机制。本综述将重点介绍蛋白质组学研究如何有助于我们理解这些领域发病机制的实例，特别强调使用 III 型和 IV 型分泌系统的病原体。此外，我们还将讨论蛋白质组学在该领域进一步研究和发现中可能发挥作用的领域。
