Omu A E, Al-Azemi M K, Omu F E, Fatinikun T, Abraham S, George S, Mahnazhath N
Department of Obstetrics and Gynaecology, Faculty of Medicine, Kuwait University, Safat, Kuwait.
J Obstet Gynaecol. 2010 Feb;30(2):122-6. doi: 10.3109/01443610903443913.
A total of: 25 women with gestational diabetes, 25 with type 2 diabetes, 21 with healthy pregnancies and 15 non-pregnant healthy controls were investigated to evaluate the relationship between butyrylcholinesterase activity and antioxidant status in the serum and placenta of diabetic pregnant women. Levels of antioxidant activities were estimated by Randox Kits and malondialdehyde and butyrylcholinesterase by colorimetric methods. Butyrylcholinesterase activity was elevated in the serum and placenta in normal pregnancy vs diabetic cohorts (p < 0.01) and there was a higher activity level in gestational and type 2 diabetes on insulin (p < 0.05) compared with diet controlled. There was higher malondialdehyde and lower antioxidant activity in diet vs insulin controlled diabetes (p < 0.01). Serum and placental butyrylcholinesterase activity showed a strong inverse correlation with malondialdehyde (r = -0.876, p < 0.001) and (r = 0.542, p < 0.01), but strong positive correlation with total antioxidant activity in serum (r = 0.764, p < 0.001) and placenta (r = 0.642, p < 0.01). Butyrylcholinesterase may therefore, be involved in reducing oxidative stress in diabetic pregnancy.
总共对25名妊娠糖尿病女性、25名2型糖尿病女性、21名正常妊娠女性和15名非妊娠健康对照者进行了调查，以评估糖尿病孕妇血清和胎盘中丁酰胆碱酯酶活性与抗氧化状态之间的关系。抗氧化活性水平通过兰多克斯试剂盒进行评估，丙二醛和丁酰胆碱酯酶通过比色法进行测定。与糖尿病组相比，正常妊娠组血清和胎盘中的丁酰胆碱酯酶活性升高（p < 0.01），与饮食控制相比，妊娠糖尿病和2型糖尿病患者使用胰岛素时的活性水平更高（p < 0.05）。饮食控制的糖尿病患者与胰岛素控制的糖尿病患者相比，丙二醛水平更高，抗氧化活性更低（p < 0.01）。血清和胎盘丁酰胆碱酯酶活性与丙二醛呈强烈负相关（r = -0.876，p < 0.001）和（r = 0.542，p < 0.01），但与血清（r = 0.764，p < 0.001）和胎盘（r = 0.642，p < 0.01）中的总抗氧化活性呈强烈正相关。因此，丁酰胆碱酯酶可能参与减轻糖尿病妊娠中的氧化应激。
