Barone Paul W, Strano Michael S
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
J Diabetes Sci Technol. 2009 Mar 1;3(2):242-52. doi: 10.1177/193229680900300204.
This article reviews current efforts to make glucose sensors based on the inherent optical properties of single walled carbon nanotubes. The advantages of single walled carbon nanotubes over traditional organic and nanoparticle fluorophores for in vivo-sensing applications are discussed. Two recent glucose sensors made by our group are described, with the first being an enzyme-based glucose sensor that couples a reaction mediator, which quenches nanotube fluorescence, on the surface of the nanotube with the reaction of the enzyme. The second sensor is based on competitive equilibrium binding between dextran-coated nanotubes and concanavalin A. The biocompatibility of a model sensor is examined using the chicken embryo chorioallantoic membrane as a tissue model. The advantages of measuring glucose concentration directly, like most optical sensors, versus measuring the flux in glucose concentration, like most electrochemical sensors, is discussed.
本文综述了目前基于单壁碳纳米管固有光学特性制备葡萄糖传感器的研究进展。讨论了单壁碳纳米管相对于传统有机荧光团和纳米颗粒荧光团在体内传感应用中的优势。描述了我们小组最近制备的两种葡萄糖传感器，第一种是基于酶的葡萄糖传感器，它将一种能淬灭纳米管荧光的反应介质与酶的反应偶联在纳米管表面。第二种传感器基于葡聚糖包被的纳米管与伴刀豆球蛋白A之间的竞争平衡结合。使用鸡胚绒毛尿囊膜作为组织模型来检测一种模型传感器的生物相容性。讨论了与大多数电化学传感器测量葡萄糖浓度通量不同，像大多数光学传感器那样直接测量葡萄糖浓度的优势。
