State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water, Environment of Ministry of Education, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
J Hazard Mater. 2010 Jun 15;178(1-3):377-84. doi: 10.1016/j.jhazmat.2010.01.090. Epub 2010 Jan 25.
Two membrane bioreactors (MBRs) with different operation conditions were employed to investigate the role of dissolved organic matter (DOM) in membrane fouling. DOM characteristics and their correlations with membrane fouling in the MBR systems were studied by using three-dimensional excitation-emission matrix (EEM) fluorescence technology, gel filtration chromatography (GFC) analysis, and column chromatographic method for DOM fractionation, etc. The three-dimensional EEM fluorescence spectroscopy analysis indicated that the fluorescence intensity of protein-like peaks in DOM samples collected from the MBR zones showed positive correlations with membrane fouling. The fluorescence spectra of membrane foulants also exhibited two protein-like peaks, confirming that proteins played an important role in membrane fouling. The DOM samples collected from MBR zones were fractionated into four components, i.e., hydrophobic (HPO), transphilic (TPI), charged hydrophilic (HPI-C) and neutral hydrophilic fractions (HPI-N). It was found that HPI-N was the most abundant fraction in all the samples, accounting for 42.0-48.9% of the total DOM. Test results also showed that HPI-N had the highest fouling potential, which could be attributed to the high molecular weight (MW) distribution and the high membrane rejection rate of macromolecules.
采用两种操作条件不同的膜生物反应器（MBR）来考察溶解有机物（DOM）在膜污染中的作用。通过三维激发-发射矩阵（EEM）荧光技术、凝胶过滤色谱（GFC）分析和柱色谱法等 DOM 分级方法，研究了 MBR 系统中 DOM 特性及其与膜污染的相关性。三维 EEM 荧光光谱分析表明，从 MBR 区采集的 DOM 样品中蛋白质类峰的荧光强度与膜污染呈正相关。膜污染物的荧光光谱也显示出两个蛋白质类峰，证实了蛋白质在膜污染中起着重要作用。从 MBR 区采集的 DOM 样品被分成四个组分，即疏水性（HPO）、迁移性（TPI）、带电亲水性（HPI-C）和中性亲水性（HPI-N）。结果发现，HPI-N 是所有样品中最丰富的组分，占总 DOM 的 42.0-48.9%。试验结果还表明，HPI-N 具有最高的污染潜力，这归因于高分子量（MW）分布和大分子的高膜截留率。
