Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute, 1 Cleantech Loop, #06-10, Singapore, 637141, Singapore.
School of Civil Engineering and Surveying, Faculty of Health, Engineering and Sciences, University of Southern Queensland, Toowoomba, 4350, Australia.
Environ Sci Pollut Res Int. 2018 Feb;25(5):4289-4302. doi: 10.1007/s11356-017-0586-x. Epub 2017 Nov 27.
In this study, the production, composition, and characteristics of dissolved organic matter (DOM) in an anoxic-aerobic submerged membrane bioreactor (MBR) were investigated. The average concentrations of proteins and carbohydrates in the MBR aerobic stage were 3.96 ± 0.28 and 8.36 ± 0.89 mg/L, respectively. After membrane filtration, these values decreased to 2.9 ± 0.2 and 2.8 ± 0.2 mg/L, respectively. High performance size exclusion chromatograph (HP-SEC) analysis indicated a bimodal molecular weight (MW) distribution of DOMs, and that the intensities of all the peaks were reduced in the MBR effluent compared to the influent. Three-dimensional fluorescence excitation emission matrix (FEEM) indicated that fulvic and humic acid-like substances were the predominant DOMs in biological treatment processes. Precise identification and characterization of low-MW DOMs was carried out using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis indicated that the highest peak numbers (170) were found in the anoxic stage, and 54 (32%) compounds were identified with a similarity greater than 80%. Alkanes (28), esters (11), and aromatics (7) were the main compounds detected. DOMs exhibited both biodegradable and recalcitrant characteristics. There were noticeable differences in the low-MW DOMs present down the treatment process train in terms of numbers, concentrations, molecular weight, biodegradability, and recalcitrance.
本研究考察了缺氧好氧浸没式膜生物反应器（MBR）中溶解性有机物（DOM）的产生、组成和特性。MBR 好氧阶段的蛋白质和碳水化合物的平均浓度分别为 3.96±0.28 和 8.36±0.89mg/L。经过膜过滤后，这些值分别降低至 2.9±0.2 和 2.8±0.2mg/L。高效尺寸排阻色谱（HP-SEC）分析表明，DOM 的分子量（MW）分布呈双峰型，与进水相比，MBR 出水中所有峰的强度均降低。三维荧光激发发射矩阵（FEEM）表明，富里酸和腐殖酸类物质是生物处理过程中主要的 DOM。采用气相色谱-质谱联用（GC-MS）对低 MW DOM 进行了精确的鉴定和表征。GC-MS 分析表明，缺氧阶段的峰数最多（170），相似度大于 80%的化合物有 54 种（32%）被鉴定。烷烃（28）、酯类（11）和芳烃（7）是检测到的主要化合物。DOM 表现出可生物降解和难降解的特性。在处理过程中，低 MW DOM 的数量、浓度、分子量、生物降解性和难降解性方面存在显著差异。
