Ishida Yojiro, Ahmed Ashraf M, Mahfouz Nadia B, Kimura Tomomi, El-Khodery Sabry A, Moawad Amgad A, Shimamoto Tadashi
Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Japan.
J Vet Med Sci. 2010 Jun;72(6):727-34. doi: 10.1292/jvms.09-0538. Epub 2010 Feb 9.
As little is known about antimicrobial resistance genes in fish farms, this study was conducted to monitor the incidence and prevalence of a wide range of antimicrobial resistance genes in Gram-negative bacteria isolated from water samples taken from fish farms in the northern part of Egypt. Ninety-one out of two hundred seventy-four (33.2%) non-repetitive isolates of Gram-negative bacteria showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing results showed that 72 (26.3%) isolates contain tetracycline resistance genes and 19 (6.9%) isolates were positive for class 1 integrons with 12 different gene cassettes. The beta-lactamase-encoding genes were identified in 14 (5.1%) isolates. The plasmid-mediated quinolone resistance genes, qnr and aac(6')-Ib-cr, were identified in 16 (5.8%) and 3 (1.1%) isolates, respectively. Finally, the florphenicol resistance gene, floR, was identified in four (1.5%) isolates. To the best of our knowledge, this is the first report for molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from fish farms in Africa.
由于对养鱼场中的抗菌药物耐药基因知之甚少，本研究旨在监测从埃及北部养鱼场采集的水样中分离出的革兰氏阴性菌中多种抗菌药物耐药基因的发生率和流行情况。在274株非重复的革兰氏阴性菌分离株中，有91株（33.2%）表现出多重耐药表型并携带至少一种抗菌药物耐药基因。聚合酶链反应（PCR）和DNA测序结果显示，72株（26.3%）分离株含有四环素耐药基因，19株（6.9%）分离株的1类整合子呈阳性，带有12种不同的基因盒。在14株（5.1%）分离株中鉴定出β-内酰胺酶编码基因。质粒介导的喹诺酮耐药基因qnr和aac(6')-Ib-cr分别在16株（5.8%）和3株（1.1%）分离株中被鉴定出来。最后，在4株（1.5%）分离株中鉴定出氟苯尼考耐药基因floR。据我们所知，这是关于非洲养鱼场分离出的革兰氏阴性菌抗菌药物耐药性分子特征的首次报告。
