Medicine B Department, Barzilai medical Center, Ben Gurion University of Negev, Ashkelon, Israel.
Am J Med Sci. 2010 Feb;339(2):117-22. doi: 10.1097/MAJ.0b013e3181c6a968.
A possible link between chronic vascular inflammation and arterial hypertension is now an object of intensive studies.
To compare Th1/Th2/Th17 cells-related cytokines, circulating endothelial progenitor cells (EPC), and endothelial function in subjects with resistant arterial hypertension (RAH) and controlled arterial hypertension (CAH).
Blood pressure was measured by electronic sphygmomanometer. EPC were identified as CD34+/CD133+/kinase insert domain receptor (KDR)+ cells by flow cytometry. Th1/Th2/Th17 cells-related cytokines were identified using the Human Th1/Th2/Th17 Cytokines MultiAnalyte ELISArray Kit. Endothelium-dependent (FMD) vasodilatation of brachial artery was measured by Doppler ultrasound scanning.
RAH group (n = 20) and CAH group (n = 20) and 17 healthy individuals (control group) were recruited. In the RAH group, lower blood levels of EPC number (42.4 +/- 16.7 cells/mL) and EPC% (0.19 +/- 0.08%) were observed than in the CAH group (93.1 +/- 88.7 cells/mL; P = 0.017; 0.27 +/- 0.17; P = 0.036) and control group (68.5 +/- 63.6 cells/mL; P < 0.001; 0.28 +/- 0.17%; P = 0.003), respectively. Plasma transforming growth factor-beta1 levels were significantly higher in the RAH group (1767 +/- 364 pg/mL) than in the CAH group (1292 +/- 349; P < 0.001) and in control group (1203 +/- 419 pg/mL; P < 0.001). In the RAH group, statistically significant negative correlation was observed between systolic blood pressure and EPC% (r = -0.72, P < 0.01). FMD in the RAH group was significantly lower (5.5 +/- 0.8%) than in the CAH group (9.2 +/- 1.4; P < 0.001) and in healthy controls (10.1 +/- 1.1%; P < 0.001).
RAH is characterized by reduced circulating EPC, substantial endothelial dysfunction, and increased plasma transforming growth factor-beta1 levels.
慢性血管炎症与动脉高血压之间的可能联系是目前正在进行的深入研究的课题。
比较难治性动脉高血压（RAH）和控制良好的动脉高血压（CAH）患者中 Th1/Th2/Th17 细胞相关细胞因子、循环内皮祖细胞（EPC）和内皮功能。
使用电子血压计测量血压。通过流式细胞术将 EPC 鉴定为 CD34+/CD133+/激酶插入结构域受体（KDR）+细胞。使用 Human Th1/Th2/Th17 Cytokines MultiAnalyte ELISArray Kit 鉴定 Th1/Th2/Th17 细胞相关细胞因子。通过多普勒超声扫描测量肱动脉内皮依赖性（FMD）血管舒张功能。
纳入了 20 名 RAH 组患者、20 名 CAH 组患者和 17 名健康个体（对照组）。在 RAH 组中，观察到 EPC 数量（42.4±16.7 个/mL）和 EPC%（0.19±0.08%）的血水平低于 CAH 组（93.1±88.7 个/mL；P=0.017；0.27±0.17；P=0.036）和对照组（68.5±63.6 个/mL；P＜0.001；0.28±0.17%；P=0.003）。RAH 组的转化生长因子-β1 水平明显高于 CAH 组（1767±364 pg/mL）和对照组（1203±419 pg/mL；P＜0.001）。在 RAH 组中，收缩压与 EPC%呈显著负相关（r=-0.72，P＜0.01）。RAH 组的 FMD 明显低于 CAH 组（9.2±1.4）和对照组（10.1±1.1%；P＜0.001）。
RAH 的特征是循环 EPC 减少、明显的内皮功能障碍和血浆转化生长因子-β1 水平升高。
