Medicine B Department, Barzilai Medical Center, Ben Gurion University of Negev, Ashkelon, Israel.
Am J Med Sci. 2010 May;339(5):415-9. doi: 10.1097/MAJ.0b013e3181d7d496.
Endothelial progenitor cells (EPC) and complement C3 are involved in the pathophysiology of arterial hypertension. C3a is the negative regulator of progenitor cells egress during their mobilization from bone marrow. Previously, higher plasma concentration of C3 was observed in resistant arterial hypertension (RAH) than in controlled arterial hypertension (CAH). Thus, we hypothesized that RAH would be associated with complement C3 activation and reduced number of circulating EPCs.
To compare C3a, C3b and their correlation with circulating EPC in subjects with RAH and CAH.
Blood pressure was measured by electronic sphygmomanometer. EPCs were identified as CD34+/CD133+/KDR+ cells by flow cytometry. C3a and C3b were determined using enzyme-linked immunosorbent assay (Quidel, CA).
: RAH group (n = 20) and CAH group (n = 20) and 17 healthy individuals (control group) were recruited. In the RAH group, C3a (858.1 +/- 70.6 microg/dL) was higher than in the CAH group (816.1 +/- 123.3 microg/dL; P < 0.001), and in the control group (751.3 +/- 98.8; P < 0.001), C3b (564.1 +/- 54.7 microg/dL) was higher than in the CAH group (490.2 +/- 58.5 microg/dL; P < 0.001). In control group (456.3 +/- 98.8; P < 0.001), statistically significant negative correlation was observed between C3a and blood levels of EPC (r = -0.523, P = 0.018); statistically significant positive correlation was observed between systolic blood pressure and blood levels of C3a (r = 0.52, P = 0.02) and between systolic blood pressure and blood levels of C3b (r = 0.57, P = 0.009).
RAH is characterized by higher levels of C3 component fragments and a negative correlation between circulating C3a and EPCs.
内皮祖细胞（EPC）和补体 C3 参与动脉高血压的病理生理学。C3a 是祖细胞从骨髓动员时流出的负调节剂。此前，在耐药性动脉高血压（RAH）中观察到的血浆 C3 浓度高于控制性动脉高血压（CAH）。因此，我们假设 RAH 与补体 C3 激活和循环 EPC 数量减少有关。
比较 RAH 和 CAH 患者中 C3a、C3b 及其与循环 EPC 的相关性。
电子血压计测量血压。通过流式细胞术将 EPC 鉴定为 CD34+/CD133+/KDR+细胞。使用酶联免疫吸附试验（Quidel，CA）测定 C3a 和 C3b。
入选 RAH 组（n=20）和 CAH 组（n=20）和 17 名健康个体（对照组）。在 RAH 组中，C3a（858.1±70.6μg/dL）高于 CAH 组（816.1±123.3μg/dL；P<0.001），高于对照组（751.3±98.8μg/dL；P<0.001），C3b（564.1±54.7μg/dL）高于 CAH 组（490.2±58.5μg/dL；P<0.001）。在对照组中（456.3±98.8μg/dL；P<0.001），C3a 与 EPC 血水平呈显著负相关（r=-0.523，P=0.018）；收缩压与 C3a 血水平呈显著正相关（r=0.52，P=0.02），与 C3b 血水平呈显著正相关（r=0.57，P=0.009）。
RAH 的特点是 C3 片段水平升高，循环 C3a 与 EPC 之间呈负相关。
