Department of Pharmaceutical Sciences, Federal University of Paraiba, João Pessoa 58059-900, PB, Brazil.
Nutrients. 2023 Jul 4;15(13):3032. doi: 10.3390/nu15133032.
Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced by endothelial progenitor cells (EPC) in hypertension. Twelve-week-old spontaneously hypertensive rats (SHR) were treated with a vehicle, carvacrol (50 or 100 mg/kg/day), or resveratrol (10 mg/kg/day) orally for four weeks. Wistar Kyoto (WKY) rats were used as the normotensive controls. Their systolic blood pressure (SBP) was measured weekly through the tail cuff. The EPCs were isolated from the bone marrow and peripherical circulation and were quantified by flow cytometry. The functionality of the EPC was evaluated after cultivation through the quantification of colony-forming units (CFU), evaluation of eNOS, intracellular detection of reactive oxygen species (ROS), and evaluation of senescence. The superior mesenteric artery was isolated to evaluate the quantification of ROS, CD34, and CD31. Treatment with carvacrol induced EPC migration, increased CFU formation and eNOS expression and activity, and reduced ROS and senescence. In addition, carvacrol reduced vascular ROS and increased CD31 and CD34 expression. This study showed that treatment with carvacrol improved the functionality of EPC, contributing to the reduction of endothelial dysfunction.
香芹酚是一种酚类单萜，具有多种生物活性，突出表现为其抗氧化和降血压能力。然而，目前鲜有证据表明其对血管再生有影响。因此，我们评估了香芹酚对高血压患者内皮祖细胞（EPC）诱导的内皮修复的调节作用。12 周龄自发性高血压大鼠（SHR）经口给予香芹酚（50 或 100mg/kg/天）或白藜芦醇（10mg/kg/天）治疗 4 周。Wistar 京都大鼠（WKY）作为正常血压对照。每周通过尾套测量其收缩压（SBP）。EPC 从骨髓和外周循环中分离出来，并通过流式细胞术进行定量。通过对集落形成单位（CFU）的定量、对 eNOS 的评估、细胞内活性氧（ROS）的检测以及衰老的评估来评估 EPC 的功能。分离肠系膜上动脉以评估 ROS、CD34 和 CD31 的定量。香芹酚治疗可诱导 EPC 迁移，增加 CFU 形成和 eNOS 表达和活性，并减少 ROS 和衰老。此外，香芹酚还降低了血管内的 ROS，并增加了 CD31 和 CD34 的表达。本研究表明，香芹酚治疗可改善 EPC 的功能，有助于减少内皮功能障碍。
