Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Parasitol Res. 2010 Apr;106(5):1055-64. doi: 10.1007/s00436-010-1744-y. Epub 2010 Feb 10.
Forensically important flesh fly species in Thailand have been investigated for their larval morphology and developmental rate to increase the forensic entomology database in Thailand and nearby countries. Sarcophaga (Liosarcophaga) dux Thomson, Sarcophaga (Liopygia) ruficornis (Fabricius), and Sarcophaga (Boettcherisca) peregrina (Robineau-Desvoidy) are the species of greatest forensic importance. The third instars of these species are similar in morphological appearance, making it difficult or impossible to differentiate between them based on external morphological characteristics. This study compares these important characteristics and reveals that the most distinctive is the posterior spiracle, followed by the number of papillae on the anterior spiracle. For confirmation of these species, the unique characteristics of adult males are also shown for accurate identification. Both the third instar and adult male characteristics are keys to identifying these species. In addition, the developmental rate of S. dux was examined in northern Thailand during 2002-2003 under natural ambient temperature (approximately 24-28 degrees C) and a natural light/dark photoperiod (approximately 12:12 h), indicating relatively rapid larval development in summer (March-June), pre-pupae (stop moving) initiated at 72 h. In the rainy season (July-October), pre-pupae initiated at 72 or 96 h, whereas pre-pupae initiated at 96 h in winter.
本研究旨在调查泰国具有法医学重要意义的肉蝇种类的幼虫形态和发育速率,以增加泰国和周边国家的法医昆虫学数据库。具有最大法医学重要性的物种包括 Sarcophaga (Liosarcophaga) dux Thomson、Sarcophaga (Liopygia) ruficornis (Fabricius) 和 Sarcophaga (Boettcherisca) peregrina (Robineau-Desvoidy)。这些物种的三龄幼虫在形态外观上相似,因此根据外部形态特征很难或不可能区分它们。本研究比较了这些重要特征,结果表明最具鉴别特征的是后气门,其次是前气门上的乳突数量。为了确认这些物种,还展示了雄性成虫的独特特征,以进行准确鉴定。三龄幼虫和雄性成虫的特征都是识别这些物种的关键。此外,还在 2002-2003 年期间在泰国北部自然环境温度(约 24-28 摄氏度)和自然光照/黑暗光周期(约 12:12 小时)下检查了 S. dux 的发育速率,表明夏季(3 月至 6 月)幼虫发育相对较快,预蛹(停止移动)在 72 小时开始。在雨季(7 月至 10 月),预蛹在 72 或 96 小时开始,而在冬季则在 96 小时开始。