Department of Pharmaceutics, University of Utah, Salt Lake City, Utah 84112, USA.
Macromol Biosci. 2010 Apr 8;10(4):445-54. doi: 10.1002/mabi.200900295.
Biodegradable hydrogels were synthesized by the click reaction of 4-arm azido-terminated PEG differing in molecular weight (2,100 and 8,800) and two alkyne-terminated peptides: [alkyne]-GFLGK-[alkyne] and ([alkyne]-GFLG)(2)K. The physical properties of in situ formed hydrogels were examined. The hydrogels were highly elastic as determined by rheological and microrheological studies. Swelling degree and enzymatic degradation by papain were dependent on the molecular weight of the PEG, but not the peptide. For PEG8800-based hydrogels, time-course analysis of degradation showed that the molecular weight of the soluble fraction quickly reached the PEG precursor value. These findings may guide future design of hydrogels with controllable mechanical properties and enzymatic degradability.
可生物降解水凝胶通过 4 臂叠氮端 PEG（分子量分别为 2100 和 8800）和两种炔基端肽的点击反应合成：[炔基]-GFLGK-[炔基]和([炔基]-GFLG)（2）K。研究了原位形成的水凝胶的物理性质。流变学和微流变学研究表明，水凝胶具有高弹性。溶胀度和木瓜蛋白酶的酶降解程度取决于 PEG 的分子量，但与肽无关。对于基于 PEG8800 的水凝胶，降解的时程分析表明，可溶性部分的分子量很快达到 PEG 前体值。这些发现可能为具有可控机械性能和酶降解性的水凝胶的未来设计提供指导。
