Department of Medicine Solna, Clinical Allergy Research Unit, Karolinska Institutet, Stockholm, Sweden.
Allergy. 2010 Oct;65(10):1282-9. doi: 10.1111/j.1398-9995.2010.02328.x. Epub 2010 Feb 6.
Environmental factors, including the intrauterine environment, can influence the risk of allergy development. In the present study, we investigated whether lifestyle and parental allergen sensitization status are reflected at gene expression level in the intrauterine environment.
mRNA expression of 17 genes was determined by means of quantitative real-time PCR in term placenta of 36 families participating in the ALADDIN study (Assessment of Lifestyle and Allergic Disease During Infancy). Data were analysed using a linear regression model to estimate the influence of lifestyle and parental allergen sensitization on the relative mRNA expression levels. Immunohistochemistry on placenta biopsies was used to verify protein expression.
Significant differences in mRNA expression levels were detected at the foetal side of the placenta, where CD14 was expressed at higher levels in placentas from families living on a farm compared to not living on a farm, and IL-12(p40) was expressed at lower levels when the father was sensitized compared to nonsensitized. At the maternal side of the placenta, higher expression of STAT4 and lower expression of GATA3 were detected in families with sensitized compared to nonsensitized mothers, and IL-12(p40) was lower expressed when the families were living on a farm compared to not living on a farm. Immunohistochemistry performed for STAT4 and GATA3 showed that protein and mRNA levels correlated well.
Living on a farm and parental allergen sensitization are reflected in the intrauterine environment at the gene expression level.
环境因素，包括宫内环境，可能会影响过敏发展的风险。在本研究中，我们调查了生活方式和父母过敏致敏状态是否反映在宫内环境的基因表达水平上。
通过定量实时 PCR 方法检测参与 ALADDIN 研究（婴儿期生活方式和过敏疾病评估）的 36 个家庭的足月胎盘中 17 个基因的 mRNA 表达。使用线性回归模型分析数据，以估计生活方式和父母过敏致敏对相对 mRNA 表达水平的影响。使用胎盘活检的免疫组织化学验证蛋白表达。
在胎盘胎儿侧检测到 mRNA 表达水平的显著差异，与不住在农场的家庭相比，来自居住在农场家庭的胎盘中 CD14 的表达水平更高，而当父亲致敏时，IL-12(p40) 的表达水平更低。在胎盘母体侧，与非致敏母亲相比，致敏母亲的 STAT4 表达水平更高，GATA3 表达水平更低，而当家庭居住在农场时，IL-12(p40) 的表达水平更低。进行的 STAT4 和 GATA3 免疫组织化学显示蛋白和 mRNA 水平具有良好的相关性。
居住在农场和父母过敏致敏状态在基因表达水平上反映在宫内环境中。
