Weisblat David A, Kuo Dian-Han
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
Cold Spring Harb Protoc. 2009 Apr;2009(4):pdb.prot5189. doi: 10.1101/pdb.prot5189.
Gravid Helobdella (leech) adults are identified by white egg masses that are visible through the ventral body wall. For convenience, gravid animals should be moved to smaller bowls so that those animals with newly laid embryos can be more readily identified. Zygotes (fertilized internally) turn pink when they are deposited into transparent cocoons on the ventral surface of the parent. A typical clutch consists of 20-100 embryos. Embryos can be collected after zygote deposition and before hatching (stage 10). This article describes how to collect and culture Helobdella embryos.
怀孕的水蛭成虫可通过其腹侧体壁可见的白色卵块来识别。为方便起见，应将怀孕的水蛭转移到较小的容器中，以便更容易识别那些已产下新胚胎的个体。受精卵（在体内受精）在被产到母体腹面的透明茧中时会变成粉红色。典型的一窝卵包含20 - 100个胚胎。胚胎可以在受精卵产下后、孵化前（第10阶段）收集。本文描述了如何收集和培养水蛭胚胎。
