蛭形轮虫（水蛭）胚胎的原位杂交
In situ hybridization of Helobdella (leech) embryos.
作者信息
Weisblat David A, Kuo Dian-Han
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
出版信息
Cold Spring Harb Protoc. 2009 Apr;2009(4):pdb.prot5194. doi: 10.1101/pdb.prot5194.
PMID:20147137
Abstract
The purpose of this protocol is to reveal the localization of transcripts in the Helobdella (leech) embryo. In situ hybridization protocols for Helobdella embryos are derived from those used for zebrafish and Xenopus. The protocols are different for early- and late-stage embryos (stages 1-8 and stages 9-11, respectively) due to a difference in tissue permeability.
摘要
本实验方案的目的是揭示水蛭胚胎中转录本的定位。水蛭胚胎的原位杂交实验方案源自用于斑马鱼和非洲爪蟾的方案。由于组织通透性的差异，早期和晚期胚胎（分别为1-8期和9-11期）的实验方案有所不同。
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