免疫染色涡虫（水蛭）胚胎。
Immunostaining Helobdella (leech) embryos.
作者信息
Weisblat David A, Kuo Dian-Han
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
出版信息
Cold Spring Harb Protoc. 2009 Apr;2009(4):pdb.prot5193. doi: 10.1101/pdb.prot5193.
The purpose of this staining protocol is to detect the localization of specific antigens in the Helobdella (leech) embryo. Immunostaining protocols must be optimized for each antibody and embryonic stage. Here, as a starting point, we present a general-purpose immunostaining protocol. Immunostaining of the leech embryo can be performed on the intact embryo or on dissected parts. For post-gastrulation embryos (stages 9 and 10), if the area of interest is in the germinal plate, immunostaining can be performed on the dissected germinal plate. It can also be performed on the ventral nerve cords dissected from late-stage embryos or juveniles.
本染色方案的目的是检测特定抗原在蛭形轮虫（水蛭）胚胎中的定位。免疫染色方案必须针对每种抗体和胚胎阶段进行优化。在此，作为一个起点，我们提供一个通用的免疫染色方案。水蛭胚胎的免疫染色可以在完整胚胎上或解剖后的部分上进行。对于原肠胚形成后的胚胎（第9和第10阶段），如果感兴趣的区域在生发板中，可以在解剖后的生发板上进行免疫染色。也可以在从晚期胚胎或幼体解剖得到的腹神经索上进行。
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