Weisblat David A, Kuo Dian-Han
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
Cold Spring Harb Protoc. 2009 Apr;2009(4):pdb.prot5191. doi: 10.1101/pdb.prot5191.
Embryos of glossiphoniid leeches are enclosed in a thin vitelline envelope until "hatching" (stage 10). This protocol describes the procedure for removing the vitelline envelope from Helobdella robusta and H. sp. (Austin) embryos. This protocol is applicable to embryos of stages 1-9 but is probably more useful for early stages. With careful culturing, the devitellinized embryos can develop normally.
舌蛭科水蛭的胚胎在“孵化”（第10阶段）之前都包裹在一层薄薄的卵黄膜中。本方案描述了从强壮扁蛭和奥斯汀扁蛭胚胎中去除卵黄膜的步骤。该方案适用于第1 - 9阶段的胚胎，但可能对早期阶段更有用。经过仔细培养，去除卵黄膜的胚胎可以正常发育。
