Gen-Probe PACE 2系统、直接荧光抗体法和细胞培养法检测宫颈标本中沙眼衣原体的比较。
Comparison of the Gen-Probe PACE 2 system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens.
作者信息
Iwen P C, Blair T M, Woods G L
机构信息
Department of Pathology, University of Nebraska Medical Center, Omaha 68198-6495.
出版信息
Am J Clin Pathol. 1991 Apr;95(4):578-82. doi: 10.1093/ajcp/95.4.578.
A chemiluminescent-labeled DNA probe (Gen-Probe PACE 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorescent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 System is a reliable method for the rapid detection of endocervical chlamydial infection.
对一种用于检测宫颈标本中沙眼衣原体的化学发光标记DNA探针（基因探针PACE 2系统）进行了评估。对每个标本都进行了组织细胞培养、直接免疫荧光染色（DFA）和DNA探针检测。318份标本中有30份（9.4%）沙眼衣原体呈阳性。与细胞培养相比，DNA探针的敏感性、特异性、阳性预测值和阴性预测值分别为93%、98%、85%和99%，而DFA的这些值分别为81%、99%、83%和99%。基因探针PACE 2系统是快速检测宫颈沙眼衣原体感染的可靠方法。
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