Ferrero D V, Meyers H N, Schultz D E, Willis S A
San Joaquin County Regional Public Health Laboratory, Stockton, California, USA.
J Clin Microbiol. 1998 Nov;36(11):3230-3. doi: 10.1128/JCM.36.11.3230-3233.1998.
The Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) uses transcription-mediated amplification and hybridization protection assay procedures to qualitatively detect Chlamydia trachomatis rRNA in urine, endocervical swab, and urethral specimens. The performance of the AMP CT was compared to that of cell culture for endocervical swab and urine specimens from women and urethral and urine specimens from men. Analysis of specimens with discrepant results was performed by a combination of reculture, direct fluorescent-antibody (DFA) staining of specimen sediment, and amplification which targeted a different chlamydial rRNA. A total of 800 urine samples were tested by the AMP CT (607 from women and 193 from men), and 7. 1% were positive for C. trachomatis, with a sensitivity of 91.2% and a specificity of 99.6% upon discrepant analysis. A total of 926 swab specimens were tested by culture and AMP CT (717 endocervical swab specimens and 209 urethral swab specimens from men), and 7.7% were positive for C. trachomatis, with a sensitivity and specificity of 100% upon discrepant analysis. The AMP CT is a sensitive and specific nucleic acid hybridization assay for the detection of C. trachomatis in endocervical swab specimens from women, urethral swab specimens from men, and urine specimens from men and women.
基因探针沙眼衣原体扩增检测法（AMPC T）采用转录介导扩增和杂交保护分析程序，对尿液、宫颈拭子及尿道标本中的沙眼衣原体rRNA进行定性检测。将AMPC T的检测性能与细胞培养法进行比较，检测对象为女性的宫颈拭子和尿液标本以及男性的尿道和尿液标本。对结果不一致的标本进行分析时，采用了再培养、标本沉淀物直接荧光抗体（DFA）染色以及针对不同衣原体rRNA的扩增相结合的方法。AMPC T共检测了800份尿液样本（607份来自女性，193份来自男性），沙眼衣原体阳性率为7.1%，经差异分析后，其灵敏度为91.2%，特异性为99.6%。共对926份拭子标本进行了培养和AMPC T检测（717份女性宫颈拭子标本和209份男性尿道拭子标本），沙眼衣原体阳性率为7.7%，经差异分析后，其灵敏度和特异性均为100%。AMPC T是一种灵敏且特异的核酸杂交检测法，可用于检测女性宫颈拭子标本、男性尿道拭子标本以及男性和女性尿液标本中的沙眼衣原体。
