Lassila Lippo V J, Garoushi Sufyan, Tanner Johanna, Vallittu Pekka K, Söderling Eva
Department of Prosthetic Dentistry & Biomaterials Science, Institute of Dentistry, University of Turku, Turku, Finland.
Open Dent J. 2009 Dec 4;3:227-32. doi: 10.2174/1874210600903010227.
OBJECTIVES.: The aim was to investigate the adhesion of Streptococcus mutans (S. mutans) to a short glass fibers reinforced semi-IPN polymer matrix composite resin. The effect of surface roughness on adhesion was also studied. For comparison, different commercial restorative materials were also evaluated. MATERIALS AND METHODS.: Experimental composite FC resin was prepared by mixing 22.5 wt% of short E-glass fibers, 22.5 wt% of IPN-resin and 55 wt% of silane treated silica fillers using high speed mixing machine. Three direct composite resins (Z250, Grandio and Nulite), resin-modified glass ionomers (Fuji II LC), amalgam (ANA 2000), fiber-reinforced composite (FRC) (everStick and Ribbond), and pre-fabricated ceramic filling insert (Cerana class 1) were tested in this study. Enamel and dentin were used as controls. The specimens (n=3/group) with or without saliva were incubated in a suspension of S. mutans allowing initial adhesion to occur. For the enumeration of cells on the disc surfaces as colony forming units (CFU) the vials with the microbe samples were thoroughly Vortex-treated and after serial dilutions grown anaerobically for 2 days at +37 degrees C on Mitis salivarius agars (Difco) containing bacitracin. Bacterial adhesion was also evaluated by using scanning electron microscopy. Surface roughness (Ra) of the materials was also determined using a surface profilometer. All results were statistically analyzed with one-way analysis of variance (ANOVA). RESULTS.: Composite FC resin and other commercial restorative materials showed similar adhesion of S. mutans, while adhesion to dentin and enamel was significantly higher (p<0.05). Surface roughness had no effect on bacterial adhesion. Saliva coating significantly decreased the adhesion for all materials (p<0.05). Composite FC resin had a significantly higher Ra value than control groups (p<0.05). CONCLUSIONS.: Short fiber-reinforced composite with semi-IPN polymer matrix revealed similar S. mutans adhesion than commercial restorative materials.
本研究旨在探究变形链球菌（S. mutans）对短玻璃纤维增强半互穿聚合物网络（semi-IPN）聚合物基复合树脂的黏附情况。同时研究表面粗糙度对黏附的影响。为作比较，还评估了不同的商用修复材料。
通过使用高速混合机将22.5 wt%的短E玻璃纤维、22.5 wt%的IPN树脂和55 wt%的硅烷处理过的二氧化硅填料混合，制备实验性复合FC树脂。本研究测试了三种直接复合树脂（Z250、Grandio和Nulite）、树脂改性玻璃离子水门汀（Fuji II LC）、汞合金（ANA 2000）、纤维增强复合材料（FRC）（everStick和Ribbond）以及预制陶瓷充填体（Cerana 1类）。以牙釉质和牙本质作为对照。将有或没有唾液覆盖的样本（每组n = 3）在变形链球菌悬液中孵育，使其发生初始黏附。为了将圆盘表面的细胞计数为菌落形成单位（CFU），将装有微生物样本的小瓶充分涡旋处理，并在连续稀释后，在含有杆菌肽的唾液链球菌琼脂（Difco）上于+37℃厌氧培养2天。还通过扫描电子显微镜评估细菌黏附情况。使用表面轮廓仪测定材料的表面粗糙度（Ra）。所有结果均采用单因素方差分析（ANOVA）进行统计分析。
复合FC树脂和其他商用修复材料对变形链球菌的黏附情况相似，而对牙本质和牙釉质的黏附显著更高（p < 0.05）。表面粗糙度对细菌黏附没有影响。唾液覆盖显著降低了所有材料的黏附力（p < 0.05）。复合FC树脂的Ra值显著高于对照组（p < 0.05）。
具有半互穿聚合物网络聚合物基体的短纤维增强复合材料对变形链球菌的黏附情况与商用修复材料相似。
