Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
J Ocul Pharmacol Ther. 2010 Feb;26(1):73-7. doi: 10.1089/jop.2009.0097.
The primary objective of this study was to examine the possibility of inhibiting oxidative damage to the lens in vitro by caffeine.
Oxidative damage was inflicted by incubating mouse lenses in Tyrode medium containing 0.1 mM Fe(8)Br(8), an iron complex soluble in aqueous medium. Parallel incubations were conducted in the presence of caffeine (5 mM).
Lenses incubated in the medium containing Fe(8)Br(8) undergo oxidative stress, as evidenced by the inhibition of Na(+)-K(+) ATPase-driven rubidium transport and the loss of tissue glutathione and ATP. These effects were prevented in presence of caffeine. That the effects are due to the oxyradicals produced was ascertained further by parallel studies with Tempol (5 mM), a well-known scavenger of reactive oxygen species (ROS) with its activity being more pronounced with hydroxyl radicals as compared to other ROS.
Caffeine was found to be effective in preventing oxidative stress to the lens induced by iron under ambient conditions. The protective effect is attributable to its ability to scavenge ROS, particularly the hydroxyl radical.
本研究的主要目的是探讨咖啡因是否能抑制体外晶状体的氧化损伤。
通过在含有 0.1mMFe(8)Br(8)的 Tyrode 培养基中孵育鼠晶状体，造成氧化损伤，Fe(8)Br(8)是一种可溶于水的铁复合物。在 5mM 咖啡因存在的情况下进行平行孵育。
在含有 Fe(8)Br(8)的培养基中孵育的晶状体会受到氧化应激，这表现在 Na(+)-K(+)ATP 酶驱动的铷转运受到抑制以及组织谷胱甘肽和 ATP 的丧失。在咖啡因存在的情况下，这些影响得到了预防。通过与 Tempol（5mM）的平行研究进一步证实了这些影响是由于产生的氧自由基所致，Tempol 是一种众所周知的活性氧（ROS）清除剂，其活性与羟基自由基相比，对其他 ROS 更为明显。
发现咖啡因能有效预防环境条件下铁引起的晶状体氧化应激。这种保护作用归因于其清除 ROS 的能力，特别是羟基自由基。
