Kaczmarczyk-Sedlak Ilona, Folwarczna Joanna, Sedlak Lech, Zych Maria, Wojnar Weronika, Szumińska Iwona, Wyględowska-Promieńska Dorota, Mrukwa-Kominek Ewa
Department of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Poland.
Department of Pharmacology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Poland.
Arch Med Sci. 2019 Jul;15(4):1073-1080. doi: 10.5114/aoms.2019.85461. Epub 2019 May 31.
One of the major causes of cataract in diabetes is oxidative stress induced by reactive oxygen species (ROS). Nowadays, new substances with antioxidative properties that may prevent cataract development are needed. One such substance is caffeine - an alkaloid with well-documented antioxidative activity.
The study was conducted on lenses obtained from female rats, divided into 3 groups: control rats; diabetic rats; diabetic rats treated with caffeine at a dose of 20 mg/kg Type 1 diabetes was induced by streptozotocin (60 mg/kg ). After 4 weeks of caffeine administration, the rats were sacrificed, and the lenses were collected, weighed and homogenized in PBS. The homogenate was used for analysis of protein content, glutathione (GSH) concentration, advanced oxidation protein product (AOPP) concentration, malondialdehyde (MDA) concentration and the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).
The SOD, CAT and GPx activities were found to be higher in the lenses of diabetic rats. There were also increased MDA and AOPP concentrations as well as decreased GSH concentration. The administration of caffeine resulted in decreased activity of SOD, CAT and GPx. The treatment with caffeine also caused an increase of GSH concentration and a decrease of MDA and AOPP concentrations.
The results of the present study may be of relevance in determining the effect of caffeine on the processes induced by ROS . Further, they can be an indication for clinical observations aiming at the assessment of both preventive and therapeutic effects of caffeine in cataract.
糖尿病性白内障的主要病因之一是活性氧（ROS）诱导的氧化应激。如今，需要具有抗氧化特性的新物质来预防白内障的发展。咖啡因就是这样一种物质——一种具有充分文献记载的抗氧化活性的生物碱。
本研究使用从雌性大鼠获取的晶状体，将其分为3组：对照大鼠；糖尿病大鼠；用20mg/kg剂量咖啡因治疗的糖尿病大鼠。通过链脲佐菌素（60mg/kg）诱导1型糖尿病。给予咖啡因4周后，处死大鼠，收集晶状体，称重并在磷酸盐缓冲盐水中匀浆。匀浆用于分析蛋白质含量、谷胱甘肽（GSH）浓度、晚期氧化蛋白产物（AOPP）浓度、丙二醛（MDA）浓度以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性。
发现糖尿病大鼠晶状体中的SOD、CAT和GPx活性较高。MDA和AOPP浓度也升高，而GSH浓度降低。给予咖啡因导致SOD、CAT和GPx活性降低。咖啡因治疗还使GSH浓度升高，MDA和AOPP浓度降低。
本研究结果可能与确定咖啡因对ROS诱导的过程的影响有关。此外，它们可为旨在评估咖啡因对白内障的预防和治疗作用的临床观察提供依据。
