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导致杏鲍菇 ITS 区 rRNA 基因直接测序时重叠峰出现的原因。

A reason for overlap peaks in direct sequencing of rRNA gene ITS in Pleurotus nebrodensis.

机构信息

State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing, China.

出版信息

FEMS Microbiol Lett. 2010 Apr;305(1):14-7. doi: 10.1111/j.1574-6968.2010.01891.x. Epub 2010 Feb 10.

DOI:10.1111/j.1574-6968.2010.01891.x
PMID:20148974
Abstract

To examine why we failed in direct sequencing of rRNA gene internal transcribed spacer (ITS) in Pleurotus nebrodensis, we obtained monokaryons of P. nebrodensis (00489 and 00491) using a protoplast monokaryonization technique. PCR products of ITS amplifications were sequenced. There was a base pair insertion/deletion difference between the two nuclei of P. nebrodensis that led to failure in direct sequencing.

摘要

为了研究我们在白灵侧耳 rRNA 基因内转录间隔区（ITS）直接测序失败的原因，我们使用原生质体单核化技术获得了白灵侧耳单核体（00489 和 00491）。对 ITS 扩增产物进行了 PCR 测序。白灵侧耳的两个核之间存在碱基对插入/缺失差异，导致直接测序失败。

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