石榴皮鞣花酸体外抗肠道病毒 71 活性。
In vitro anti-enterovirus 71 activity of gallic acid from Woodfordia fruticosa flowers.
机构信息
Jeonnam Institute of Natural Resources Research, Gisan-ri, Anyang-myeon, Jangheung-gun, Jeollanamdo, South Korea.
出版信息
Lett Appl Microbiol. 2010 Apr;50(4):438-40. doi: 10.1111/j.1472-765X.2010.02805.x. Epub 2010 Jan 22.
AIMS
The anti-enterovirus 71 (EV71) activity of six Nepalese plants' extracts and gallic acid (GA) isolated from Woodfordia fruticosa Kurz (family; Lythaceae) flowers were evaluated in Vero cells.
METHODS AND RESULTS
The anti-EV71 activity of tested compounds was evaluated by a cytopathic effect reduction method. Our results demonstrated that flowers' extracts of W. fruticosa exerted strong anti-EV71 activity, with a 50% inhibitory concentration (IC(50)) of 1.2 microg ml(-1) and no cytotoxicity at a concentration of 100 microg ml(-1), and the derived therapeutic index (TI) was more than 83.33. Rivabirin showed no antiviral activity against EV71. Furthermore, GA isolated from W. fruticosa flowers exhibited a higher anti-EV71 activity than the extract of W. fruticosa flowers, with an IC(50) of 0.76 microg ml(-1) and no cytotoxicity at a concentration of 100 microg ml(-1), and the derived TI was 99.57.
CONCLUSIONS
This study demonstrated that flower extracts of W. fruticosa possessed anti-EV71 activity and GA isolated from these flowers showed stronger anti-EV71 activity than that the extracts.
SIGNIFICANCE AND IMPACT OF THE STUDY
Our results suggest that the GA from W. fruticosa flowers may be used as a potential antiviral agent.
目的
评估来自尼泊尔六种植物提取物和从武靴藤（夹竹桃科）花中分离得到的没食子酸（GA）对肠道病毒 71 型（EV71）的抗病毒活性。
方法和结果
采用细胞病变抑制法评估了受试化合物的抗 EV71 活性。结果表明，武靴藤花提取物对 EV71 具有较强的抑制活性，其 50%抑制浓度（IC50）为 1.2μg/ml，在 100μg/ml 浓度下无细胞毒性，治疗指数（TI）大于 83.33。利巴韦林对 EV71 无抗病毒活性。此外，从武靴藤花中分离得到的 GA 对 EV71 的抗病毒活性高于武靴藤花提取物，其 IC50 为 0.76μg/ml，在 100μg/ml 浓度下无细胞毒性，TI 为 99.57。
结论
本研究表明，武靴藤花提取物具有抗 EV71 活性，而从这些花中分离得到的 GA 对 EV71 的抑制活性强于提取物。
意义和影响
我们的研究结果表明，武靴藤花中的 GA 可能被用作一种有潜力的抗病毒药物。
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