Dipartimento di Scienze Farmaceutiche, Università di Trieste, Piazzale Europa 1, 34127 Trieste, Italy.
Trends Biotechnol. 2010 Apr;28(4):171-80. doi: 10.1016/j.tibtech.2010.01.001. Epub 2010 Feb 10.
Enzymes and whole cells are being increasingly applied in research and industry, but the adoption of biocatalysis relies strongly on useful scientific literature. Unfortunately, too many published papers lack essential information needed to reproduce and understand the results. Here, members of the scientific committee of the European Federation of Biotechnology Section on Applied Biocatalysis (ESAB) provide practical guidelines for reporting experiments. The document embraces the recommendations of the STRENDA initiative (Standards for Reporting Enzymology Data) in the context of pure enzymology and provides further guidelines and explanations on topics of crucial relevance for biocatalysis. In particular, guidelines are given on issues such as the selectivity, specificity, productivity and stability of biocatalysts, as well as on methodological problems related to reactions in multiphase systems. We believe that adoption and use of these guidelines could greatly increase the value and impact of published work in biocatalysis, and hence promote the further growth of applications.
酶和完整细胞越来越多地应用于研究和工业中，但生物催化的采用强烈依赖于有用的科学文献。不幸的是，太多已发表的论文缺乏重现和理解结果所需的基本信息。在这里，生物技术欧洲联合会应用生物催化分会（ESAB）科学委员会的成员为实验报告提供了实用指南。该文件在纯酶学的背景下包含了 STRENDA 倡议（酶学数据报告标准）的建议，并就生物催化中至关重要的主题提供了进一步的指南和解释。特别是，提供了关于生物催化剂选择性、特异性、生产力和稳定性等问题的指南，以及与多相系统中反应相关的方法学问题的指南。我们相信，采用和使用这些指南可以大大提高生物催化领域已发表工作的价值和影响力，从而促进应用的进一步发展。
