Department of Biology and Evolution, University of Ferrara, via L Borsari 46, I-44100 Ferrara, FE, Italy.
J Plant Physiol. 2010 Jul 15;167(11):928-32. doi: 10.1016/j.jplph.2010.01.013. Epub 2010 Feb 10.
Phenylalanine ammonia lyase (PAL)-specific activity levels were measured in suspension-cultured cells of six rice cultivars following treatment with cell wall hydrolysates prepared from seven Pyricularia grisea strains. Early after elicitation, even low hydrolysate concentrations were able to induce a significant increase in enzyme levels. However, neither rice genotypes showing differential sensitivity to blast reacted differently, nor did elicitors obtained from various pathotypes induce different reactions. At a later stage, higher hydrolysate concentrations were required to trigger maximal enzyme induction. Also in this case, only slight variations were detected in suspension cultures of a given cultivar treated with different elicitors. On the contrary, highly significant differences were observed among plant genotypes. A clear relationship was evident between the mean increase in PAL activity and the overall resistance to blast at the plant level. This trait could therefore represent a useful tool in selection for increased blast tolerance.
苯丙氨酸解氨酶（PAL）活性水平在悬浮培养的六个水稻品种细胞中进行了测量，这些细胞经过了来自七个稻瘟病菌株的细胞壁水解产物处理。在诱导的早期，即使低浓度的水解产物也能显著诱导酶水平的增加。然而，对稻瘟病表现出不同敏感性的水稻基因型没有表现出不同的反应，也没有来自不同致病型的诱导物诱导出不同的反应。在后期，需要更高的水解产物浓度才能引发最大的酶诱导。在使用不同诱导物处理的给定品种的悬浮培养物中，也只检测到轻微的变化。相反，在植物基因型之间观察到了非常显著的差异。PAL 活性的平均增加与植物水平上对稻瘟病的总体抗性之间存在明显的关系。因此，该性状可以作为选择提高稻瘟病抗性的有用工具。
