Department of Life Science and Biotechnology, University of Ferrara, Ferrara, Italy.
Plant Biol (Stuttg). 2014 Nov;16(6):1127-32. doi: 10.1111/plb.12165. Epub 2014 Feb 12.
Intracellular free amino acid pools were quantified in suspension cultured cells of a blast-sensitive and a blast-resistant rice genotype at increasing times after treatment with Magnaporthe oryzae cell wall hydrolysates. Besides some expected variations in free phenylalanine, a remarkable early increase of γ-aminobutyric acid (GABA) levels was evident in both cultivars. Glutamate decarboxylase activity and protein levels were unaffected. GABA homeostasis was recovered in the sensitive cultivar 48 h after the treatment. In contrast, a further GABA accumulation and a general increase of most amino acids was found at this later stage in the resistant genotype, which showed a larger decrease in cell viability as a consequence of elicitor addition. Data support a recently hypothesised role of GABA metabolism in the plant response to fungal pathogens.
在与稻瘟病菌细胞壁水解产物处理后不同时间，悬浮培养的感病和抗病水稻基因型细胞内游离氨基酸库被定量。除游离苯丙氨酸的一些预期变化外，两种品种中γ-氨基丁酸（GABA）水平明显早期增加。谷氨酸脱羧酶活性和蛋白水平不受影响。敏感品种在处理后 48 小时 GABA 稳态得到恢复。相比之下，在抗性品种中，在这个后期阶段发现 GABA 进一步积累和大多数氨基酸普遍增加，这是由于添加诱导剂导致细胞活力下降更大。数据支持 GABA 代谢在植物对真菌病原体反应中的作用的最新假说。
