壳聚糖瓜尔胶纳米粒子的制备及其对水稻病原菌的体外抗菌活性。

Chitosan guar nanoparticle preparation and its in vitro antimicrobial activity towards phytopathogens of rice.

机构信息

Department of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu 24, India.

出版信息

Int J Biol Macromol. 2020 Jun 15;153:297-304. doi: 10.1016/j.ijbiomac.2020.03.001. Epub 2020 Mar 2.

DOI:10.1016/j.ijbiomac.2020.03.001

Abstract

The aim of the present study was to prepare chitosan guar nanoparticle (CGNP) with high antimicrobial activity to use as a bioprotectant against rice phytopathogens. Nanoparticles were prepared using sodium tripolyphosphate by the ionic gelation method. The physico-chemical properties of nanoparticles were characterized through DLS, FTIR, TEM, SEM, AFM and XRD. The application of CGNP to rice seeds stimulated seed germination and seedling growth. CGNP showed growth inhibition towards rice pathogens P. grisea and X. oryzae under in-vitro condition. Excised rice leaves treated with CGNP and challenged with P. grisea showed no blast disease symptom whereas control leaves showed very high blast disease symptom. The results of this study indicate that CGNP can be used as an antimicrobial agent to control blast, blight disease of rice.

摘要

本研究旨在制备具有高抗菌活性的壳聚糖瓜尔胶纳米粒子（CGNP），用作水稻病原菌的生物保护剂。纳米粒子采用三聚磷酸钠通过离子凝胶法制备。通过 DLS、FTIR、TEM、SEM、AFM 和 XRD 对纳米粒子的理化性质进行了表征。CGNP 应用于水稻种子可刺激种子萌发和幼苗生长。CGNP 在体外条件下对水稻病原菌稻瘟病菌和稻黄单胞菌表现出生长抑制作用。用 CGNP 处理并受到稻瘟病菌挑战的离体水稻叶片没有出现稻瘟病症状，而对照叶片则出现非常高的稻瘟病症状。本研究结果表明，CGNP 可用作抗菌剂来控制水稻稻瘟病和稻疫病。