Department of Medical Biophysics, University of Western Ontario, Ontario, Canada.
J Nucl Med. 2010 Mar;51(3):413-7. doi: 10.2967/jnumed.109.069732. Epub 2010 Feb 11.
Neither intravenous nor intracoronary routes provide targeted stem cell delivery to recently infarcted myocardium in sufficient quantities. Direct routes appear preferable. However, most prior studies have used epicardial injections, which are not practical for routine clinical use. The objective of this study was to compare cell retention and clearance kinetics between a subepicardial and a subendocardial technique.
We evaluated 7 dogs with each technique, using (111)In-tropolone-labeled endothelial progenitor cells and serial SPECT/CT for 15 d after injection.
In vivo indium imaging demonstrated comparable degrees of retention: 57% +/- 15% for the subepicardial injections and 54% +/- 26% for the subendocardial injections. Clearance half-lives were also similar at 69 +/- 26 and 60 +/- 21 h, respectively.
This study demonstrates that subendocardial injections, clinically more practical, show clearance kinetics comparable to those of subepicardial injections and will facilitate the ultimate clinical use of this treatment modality.
比较心外膜和心内膜途径注射细胞后的细胞保留和清除动力学。
我们用（111）In-替普隆标记的内皮祖细胞评估了 7 只狗，注射后 15 天用 SPECT/CT 连续检测。
体内铟显像显示两种途径的保留程度相似：心外膜注射为 57% +/- 15%，心内膜注射为 54% +/- 26%。清除半衰期也相似，分别为 69 +/- 26 和 60 +/- 21 h。
这项研究表明，临床更实用的心内膜注射具有与心外膜注射相似的清除动力学，将促进这种治疗方式的最终临床应用。
