Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama 227-8501, Japan.
Anticancer Res. 2010 Jan;30(1):107-9.
Deleted in colorectal carcinoma (DCC), one of the Netrin-1 receptors, belongs to the functional dependence receptor family, members of which share the ability to induce apoptosis in the absence of their ligands. Recently, we examined the methylation status of the DCC gene in colorectal carcinomas and found that aberrant methylation of the DCC gene was detected in 28 out of the 50 (56%) primary colon carcinomas. This result prompted us to examine the methylation status of the DCC gene in gastric carcinoma.
The methylation status of the DCC gene was examined in primary carcinomas and the corresponding normal tissues derived from 36 patients with gastric cancer using quantitative methylation-specific polymerase chain reaction (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.
Aberrant methylation of the DCC gene was detected in 16 out of the 36 (44%) primary gastric carcinomas. A significant difference was observed in regard to the TNM stage (p=0.0093).
DCC methylation was observed in the course of gastric carcinogenesis and disappeared in advanced gastric carcinoma.
缺失于大肠癌（DCC）中的一种神经纤毛蛋白-1 受体属于功能依赖性受体家族，该家族成员在缺乏配体的情况下具有诱导细胞凋亡的能力。最近，我们检测了结直肠癌中 DCC 基因的甲基化状态，发现 50 例原发性结肠癌中有 28 例（56%）存在 DCC 基因的异常甲基化。这一结果促使我们检测胃癌中 DCC 基因的甲基化状态。
采用定量甲基化特异性聚合酶链反应（qMSP）检测 36 例胃癌患者的原发性癌组织及其相应的正常组织中 DCC 基因的甲基化状态，并评估其甲基化状态与临床病理特征之间的相关性。
在 36 例原发性胃癌中，有 16 例（44%）存在 DCC 基因的异常甲基化。在 TNM 分期方面存在显著差异（p=0.0093）。
DCC 甲基化发生在胃癌发生过程中，并在晚期胃癌中消失。
