Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, 600 Yishan RD, Shanghai 200233, China.
J Cancer Res Clin Oncol. 2010 Oct;136(10):1489-96. doi: 10.1007/s00432-010-0806-y. Epub 2010 Feb 12.
To examine the expressions of focal adhesion kinase (FAK) and its clinical significance in hepatocellular carcinoma (HCC).
We determined the expression levels of FAK on both steady-state mRNA and protein levels in 50 HCC samples by quantitative real-time PCR and immunohistochemistry, respectively. The correlations between FAK expression and various clinicopathological parameters were analyzed.
The expression of FAK on the mRNA level was consistent with that on the protein level. FAK mRNA levels in tumor tissues were significantly higher than in the paratumor tissues (0.229 +/- 0.027 vs. 0.163 +/- 0.019; P < 0.001), but lower than in the macroscopic cancer emboli (0.506 +/- 0.155 vs. 0.377 +/- 0.176; P < 0.05). Compared within the tumor tissues, FAK expressions were significantly higher in those with cancer emboli than those without (0.343 +/- 0.05 vs. 0.165 +/- 0.025; P = 0.003). Univariate and multivariate analyses revealed that FAK expression was an independent prognostic factor for disease-free survival and overall survival. Both 3-year disease-free survival rates and overall survival rates in FAK-negative group were significantly higher than in positive group (52 vs. 20% and 72 vs. 29%, P < 0.001).
Our results suggest that FAK expression is up-regulated in HCC and its expression is an independent prognostic factor for HCC.
检测黏着斑激酶（FAK）在肝细胞癌（HCC）中的表达及其临床意义。
采用实时定量 PCR 和免疫组化法分别检测 50 例 HCC 组织中 FAK 的稳定态 mRNA 和蛋白表达水平，分析 FAK 表达与各临床病理参数的相关性。
FAK 的 mRNA 水平与蛋白水平表达一致。肿瘤组织中的 FAK mRNA 水平明显高于癌旁组织（0.229±0.027 比 0.163±0.019，P＜0.001），但低于宏观癌栓（0.506±0.155 比 0.377±0.176，P＜0.05）。肿瘤组织中，有癌栓的 FAK 表达明显高于无癌栓的（0.343±0.05 比 0.165±0.025，P=0.003）。单因素和多因素分析显示，FAK 表达是无病生存率和总生存率的独立预后因素。FAK 阴性组的 3 年无病生存率和总生存率明显高于阳性组（52%比 20%和 72%比 29%，P＜0.001）。
FAK 在 HCC 中呈高表达，其表达是 HCC 的独立预后因素。
