Center of Experimental and Applied Cutaneous Physiology CCP, Department of Dermatology and Allergology, Charité-Universitätsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany.
J Biophotonics. 2011 Jan;4(1-2):21-9. doi: 10.1002/jbio.200900111. Epub 2010 Feb 11.
Production of free radicals in the human skin subsequent to IR irradiation has been demonstrated by means of two different methods. The first technique, based on resonance Raman spectroscopy, enables the non-invasive measurements of the kinetics of cutaneous carotenoid antioxidants beta-carotene and lycopene, subsequent to IR irradiation. Obtained degradation of the cutaneous carotenoids was a hint but not evidence that IR irradiation can produce free radicals in the skin. Therefore, the direct observation sustaining the production of free radicals subsequent to IR irradiation in the skin was performed in-vitro by electron paramagnetic resonance spectroscopy. Enzymatic processes as well as heat shock-induced radicals in the human skin are presumably involved in the energy transfer from IR irradiation into the molecules of the skin. Protection strategy for human skin against IR-induced free radicals based on the increase in the concentration of antioxidants by means of antioxidant-rich supplementation is discussed.
已经通过两种不同的方法证明了人类皮肤在红外线照射后会产生自由基。第一种技术基于共振拉曼光谱学，可以对皮肤类胡萝卜素抗氧化剂β-胡萝卜素和番茄红素在红外线照射后的动力学进行非侵入性测量。皮肤类胡萝卜素的降解只是表明，而不是证明，红外线照射可以在皮肤中产生自由基。因此，通过电子顺磁共振波谱学在体外直接观察到了红外线照射后皮肤中自由基的产生。人类皮肤中的酶促过程和热休克诱导的自由基可能参与了将红外线照射的能量转移到皮肤分子中。讨论了一种基于通过富含抗氧化剂的补充剂增加抗氧化剂浓度来保护人体皮肤免受红外线诱导自由基侵害的策略。
