Department of Physics, Indian Institute of Technology Madras, Alternative Energy and Nanotechnology Laboratory, Nano Functional Materials Technology Centre, Chennai, India.
Talanta. 2010 Mar 15;80(5):2016-22. doi: 10.1016/j.talanta.2009.11.010. Epub 2009 Nov 10.
A new type of amperometric glucose biosensor based on silicon dioxide coated magnetic nanoparticle decorated multiwalled carbon nanotubes (Fe(3)O(4)@SiO(2)/MWNTs) on a glassy carbon electrode (GCE) has been developed. MWNTs have been synthesized by catalytic chemical vapour decomposition (CCVD) of acetylene over rare earth (RE) based AB(3) alloy hydride catalyst. The as-grown MWNTs have been purified and further functionlized. Functionalized MWNTs have been decorated with magnetic Fe(3)O(4) nanoparticles which have been uniformly coated with biocompatible SiO(2) using a simple chemical reduction method. The characterization of magnetic nanoparticle modified MWNTs have been done by X-ray diffraction (XRD), Fourier transform infra red spectroscopy (FT-IR), scanning electron microscope (SEM), transmission electron microscope (TEM), vibrating sample magnetometer (VSM), energy dispersive X-ray analysis (EDX) and UV-vis spectroscopy. Amperometric biosensor has been fabricated by the deposition of glucose oxidase (GOD) over Nafion-solubilized Fe(3)O(4)@SiO(2)/MWNTs electrode. The resultant bioelectrode retains its biocatalytic activity and offers fast and sensitive glucose quantification. The performance of the biosensor has been studied using cyclic voltammetry and amperometry and the results have been discussed. The fabricated glucose biosensor exhibits a linear response from 1 microM to 30 mM with an excellent detection limit of 800 nM indicating the potential applications in food industries.
一种新型的基于二氧化硅涂层磁性纳米粒子修饰多壁碳纳米管（Fe3O4@SiO2/MWNTs）的安培型葡萄糖生物传感器已被开发出来。MWNTs 是通过在稀土（RE）基 AB3 合金氢化物催化剂上催化化学气相分解（CCVD）乙炔合成的。生长的 MWNTs 已经被纯化和进一步功能化。功能化的 MWNTs 已经用磁性 Fe3O4 纳米粒子修饰，这些纳米粒子已经通过简单的化学还原方法均匀地涂覆了生物相容性的 SiO2。磁性纳米粒子修饰的 MWNTs 的表征是通过 X 射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、振动样品磁强计（VSM）、能量色散 X 射线分析（EDX）和紫外可见光谱进行的。安培型生物传感器是通过将葡萄糖氧化酶（GOD）沉积在 Nafion 溶解的 Fe3O4@SiO2/MWNTs 电极上制成的。所得的生物电极保留了其生物催化活性，并提供了快速和敏感的葡萄糖定量。使用循环伏安法和安培法研究了生物传感器的性能，并对结果进行了讨论。所制备的葡萄糖生物传感器在 1 microM 到 30 mM 的范围内呈现线性响应，具有出色的检测限 800 nM，表明其在食品工业中有潜在的应用。
