Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-sen University, Guangzhou 510060, China.
J Cataract Refract Surg. 2010 Feb;36(2):208-14. doi: 10.1016/j.jcrs.2009.08.020.
To determine the role of anterior capsule polishing in residual lens epithelial cell (LEC) proliferation.
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
Phacoemulsification was performed on pairs of human cadaver eyes. One eye of each pair had anterior capsule polishing; the fellow eye did not. Capsular bags with implanted intraocular lenses (IOLs) were incubated in culture. Specimens were observed and photographed under microscopy. For clinical comparison, both eyes of patients who had phacoemulsification and IOL implantation with anterior capsule polishing in 1 eye only were observed for 1 year postoperatively.
The study comprised 10 pairs of cadaver eyes and 20 eyes of 10 patients. One day after surgery, large patches of cells remained under the anterior capsule and the equatorial zone in the unpolished cadaver eyes and the anterior capsule was clear in the polished eyes. By 3 days in culture, many patches of dead cells had formed in the unpolished eyes. After 7 days in culture, cell growth was minimal in the unpolished eyes; however, robust cell proliferation was observed in the polished eyes. In the clinical comparison, there was no obvious difference in the mean subjectively assessed fibrotic posterior capsule opacification (PCO) score between polished eyes and unpolished eyes at 1 year.
Anterior capsule polishing, although it removed many LECs, did not decrease residual cell growth and, conversely, enhanced cell proliferation in capsular bag cultures. This might explain why polishing does not reduce PCO in clinical studies.
确定前囊抛光术在残留晶状体上皮细胞（LEC）增殖中的作用。
中国广州中山大学中山眼科中心。
对成对的人尸体眼球进行超声乳化术。每只眼球的一只眼进行前囊抛光，另一只眼不进行抛光。将植入人工晶状体（IOL）的囊袋在培养箱中孵育。在显微镜下观察和拍照标本。为了进行临床比较，对仅在一只眼行超声乳化术和 IOL 植入术并在前囊抛光的 10 例患者的双眼进行了 1 年的术后观察。
本研究包括 10 对尸体眼球和 20 只眼。术后 1 天，未抛光的尸体眼球的前囊和赤道区下方仍有大片细胞，而抛光后的前囊则清晰可见。培养 3 天后，未抛光眼的许多前囊下形成了大片死细胞。培养 7 天后，未抛光眼的细胞生长很少，但抛光眼则观察到明显的细胞增殖。在临床比较中，抛光眼和未抛光眼在 1 年时的主观评估后发性白内障（PCO）纤维膜混浊评分均值无明显差异。
虽然前囊抛光术去除了许多 LEC，但并未减少残留细胞的生长，反而增强了囊袋培养中的细胞增殖。这可能解释了为什么在临床研究中抛光术不能减少 PCO。
