Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, PR China.
J Pharm Biomed Anal. 2010 Aug 1;52(4):625-30. doi: 10.1016/j.jpba.2010.01.035. Epub 2010 Jan 28.
An LC-MS/MS method was developed for the simultaneous determination of chlorogenic acid, forsythiaside A and baicalin, three major ingredients in Shuang-huang-lian preparations, in rat plasma. Following extraction by methanol-ethyl acetate-trifluoroacetic acid (49:49:2, v/v/v), the extracted analytes were separated on a reverse phase C12 column using a gradient mobile phase system of acetonitrile-water containing 0.1% formic acid. The limits of quantification were between 1.0 and 2.1ng/mL, the precision was <7% and the accuracy was between 94% and 107%. The validated method was applied to a comparative pharmacokinetic study in rats after administration of Shuang-huang-lian solutions via intravenous, peroral or intratracheal routes. The results showed that the three chemical markers were more rapidly and thoroughly absorbed following pulmonary delivery as compared with peroral administration.
建立了一种 LC-MS/MS 法，用于同时测定大鼠血浆中三种主要成分——绿原酸、连翘酯苷 A 和黄芩苷——的含量。样品经甲醇-乙酸乙酯-三氟乙酸（49:49:2，v/v/v）提取后，采用含 0.1%甲酸的乙腈-水为流动相进行梯度洗脱，在反相 C12 柱上进行分离。该方法的定量下限为 1.02.1ng/mL，精密度<7%，准确度在 94%107%之间。该方法经验证后，用于比较双黄连溶液经静脉、口服和气管内给药后在大鼠体内的药代动力学。结果表明，与口服给药相比，肺部给药后三种化学标志物的吸收更快、更完全。
