Inp2在过氧化物酶体遗传中的保守功能。

A conserved function for Inp2 in peroxisome inheritance.

作者信息

Saraya Ruchi, Cepińska Małgorzata N, Kiel Jan A K W, Veenhuis Marten, van der Klei Ida J

机构信息

Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, AA Haren, The Netherlands.

出版信息

Biochim Biophys Acta. 2010 May;1803(5):617-22. doi: 10.1016/j.bbamcr.2010.02.001. Epub 2010 Feb 11.

DOI:10.1016/j.bbamcr.2010.02.001

PMID:20153784

Abstract

In budding yeast Saccharomyces cerevisiae, the peroxisomal protein Inp2 is required for inheritance of peroxisomes to the bud, by connecting the organelles to the motor protein Myo2 and the actin cytoskeleton. Recent data suggested that the function of Inp2 may not be conserved in other yeast species. Using in silico analyses we have identified a weakly conserved Inp2-related protein in 18 species of budding yeast and analyzed the role of the identified protein in the methylotrophic yeast Hansenula polymorpha in peroxisome inheritance. Our data show that H. polymorpha Inp2 locates to peroxisomes, interacts with Myo2, and is essential for peroxisome inheritance.

摘要

在出芽酵母酿酒酵母中，过氧化物酶体蛋白Inp2通过将细胞器与驱动蛋白Myo2和肌动蛋白细胞骨架相连，对于过氧化物酶体遗传至芽体是必需的。最近的数据表明，Inp2的功能在其他酵母物种中可能并不保守。通过计算机分析，我们在18种出芽酵母中鉴定出一种弱保守的Inp2相关蛋白，并分析了所鉴定蛋白在甲基营养型酵母多形汉逊酵母过氧化物酶体遗传中的作用。我们的数据表明，多形汉逊酵母Inp2定位于过氧化物酶体，与Myo2相互作用，并且对于过氧化物酶体遗传至关重要。