Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
Cancer Epidemiol. 2010 Apr;34(2):157-61. doi: 10.1016/j.canep.2009.12.016. Epub 2010 Feb 13.
The number of women infected with human papillomavirus (HPV) and the distribution of the HPV genotypes vary across populations and with age.
To determine the prevalence and genotype distribution of HPV in young married women aged 16-24 years.
1300 women residing in an urban slum in Delhi donated samples of exfoliated cervical cells that were collected by the Digene((R)) kit and tested for the presence of HPV DNA by two techniques in parallel, i.e., PCR using PGMY consensus primers for all HPV types and the Digene HPV test (Hybrid Capture 2 (HC2) Probe B for high-risk (hr) types. Genotyping was done on all HPV positive samples using the Roche reverse line blot assay.
HPV infection was detected in 91/1300 (7%) samples by PCR and 110/1300 (8.4%) samples by HC2. Genotyping identified 20 high-risk and 11 low-risk types. HPV16 was the commonest high-risk type (3%) followed by HPV52 (1.2%) and HPV51 (0.8%). Among low-risk types, HPV62 was the commonest (0.8%), followed by HPV84 and HPV89 (0.5% each). Multiple infections were found in 3% of the HPV positive samples.
A wide spectrum of HPV genotypes is seen in this young population. Knowledge about HPV types prevalent in communities in different regions of India would be useful in devising the optimum strategy for cervical cancer prevention.
不同人群和年龄段的人感染人乳头瘤病毒（HPV）的人数和 HPV 基因型分布有所不同。
确定 16-24 岁年轻已婚妇女中 HPV 的流行率和基因型分布。
1300 名居住在德里市区贫民窟的妇女提供了脱落的宫颈细胞样本，这些样本通过 Digene((R))试剂盒收集，并通过两种平行技术检测 HPV DNA 的存在，即使用 PGMY 共识引物检测所有 HPV 类型的 PCR 和 Digene HPV 检测（HC2 探针 B 用于高危（hr）类型。对所有 HPV 阳性样本进行基因分型，使用罗氏反向线印迹分析。
PCR 检测到 91/1300（7%）样本存在 HPV 感染，HC2 检测到 110/1300（8.4%）样本存在 HPV 感染。基因分型鉴定出 20 种高危型和 11 种低危型。HPV16 是最常见的高危型（3%），其次是 HPV52（1.2%）和 HPV51（0.8%）。在低危型中，HPV62 最常见（0.8%），其次是 HPV84 和 HPV89（0.5%）。3%的 HPV 阳性样本存在多重感染。
在这个年轻人群中可以看到广泛的 HPV 基因型。了解印度不同地区社区中流行的 HPV 类型将有助于制定最佳的宫颈癌预防策略。
