Kaur Parminder, Aggarwal Aruna, Nagpal Madhu, Oberoi Loveena, Sharma Swati
Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, India ; H. No. 1658, Phase 7, Mohali, 160062 Punjab India.
Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, India.
J Obstet Gynaecol India. 2014 Aug;64(4):279-83. doi: 10.1007/s13224-014-0508-5. Epub 2014 Apr 12.
Cervical cancer is the commonest cancer among Indian women. High-risk human papilloma virus (HPV) detection holds the potential to be used as a tool to identify women, at risk of subsequent development of cervical cancer. There is a pressing need to identify prevalence of asymptomatic cervical HPV infection in local population. In our study, we explored the prevalence of HPV genotypes and their distribution in women with cervical lesions.
Scrape specimens were obtained from 100 women (study group) with cervical abnormalities. HPV was detected with amplicor HPV tests, and the individual genotypes in these specimens were identified by Hybribio Genoarray test kit. Fifty specimens were also collected from females with healthy cervix (control group). The present study also aimed to determine the status of HPV prevalence and its association with different sociodemographic factors.
Out of the total number of 100 samples, 10 (10 %) women tested positive for HPV DNA. Among them, HPV 18 was observed in 6, HPV 16 in 2, HPV 52 and HPV 39 in one each. Fifty specimens collected from patients with healthy cervix were not infected with any of the HPV genotype.
Our study generates data of HPV prevalence in patients with cervical lesions visiting tertiary care institute. The data generated will be useful for laying guidelines for mass screening of HPV detection, treatment, and prophylaxis.
宫颈癌是印度女性中最常见的癌症。高危型人乳头瘤病毒（HPV）检测有潜力作为一种工具，用于识别有后续患宫颈癌风险的女性。迫切需要确定当地人群中无症状宫颈HPV感染的患病率。在我们的研究中，我们探讨了HPV基因型的患病率及其在宫颈病变女性中的分布情况。
从100名宫颈异常的女性（研究组）中获取刮片标本。采用Amplicor HPV检测法检测HPV，并使用Hybribio基因芯片检测试剂盒鉴定这些标本中的个体基因型。还从宫颈健康的女性（对照组）中收集了50份标本。本研究还旨在确定HPV患病率的状况及其与不同社会人口学因素的关联。
在总共100份样本中，10名（10%）女性HPV DNA检测呈阳性。其中，6名检测出HPV 18型，2名检测出HPV 16型，1名分别检测出HPV 52型和HPV 39型。从宫颈健康患者中收集的50份标本未感染任何HPV基因型。
我们的研究得出了在三级医疗机构就诊的宫颈病变患者中HPV患病率的数据。所产生的数据将有助于制定HPV检测、治疗和预防的大规模筛查指南。
