Molecular Epidemiology and Veterinary Public Health Laboratory, Hopkirk Research Institute, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Appl Environ Microbiol. 2010 Apr;76(7):2145-54. doi: 10.1128/AEM.00862-09. Epub 2010 Feb 12.
In New Zealand the number of campylobacteriosis notifications increased markedly between 2000 and 2007. Notably, this country's poultry supply is different than that of many developed countries as the fresh and frozen poultry available at retail are exclusively of domestic origin. To examine the possible link between human cases and poultry, a sentinel surveillance site was established to study the molecular epidemiology of Campylobacter jejuni over a 3-year period from 2005 to 2008 using multilocus sequence typing. Studies showed that 60.1 to 81.4% of retail poultry carcasses from the major suppliers were contaminated with C. jejuni. Differences were detected in the probability and level of contamination and the relative frequency of genotypes for individual poultry suppliers and humans. Some carcasses were contaminated with isolates belonging to more than one sequence type (ST), and there was evidence of both ubiquitous and supplier-associated strains, an epidemiological pattern not recognized yet in other countries. The common poultry STs were also common in human clinical cases, providing evidence that poultry is a major contributor to human infection. Both internationally rare genotypes, such as ST-3069 and ST-474, and common genotypes, such as ST-45 and ST-48, were identified in this study. The dominant human sequence type in New Zealand, ST-474, was found almost exclusively in isolates from one poultry supplier, which provided evidence that C. jejuni has a distinctive molecular epidemiology in this country. These results may be due in part to New Zealand's geographical isolation and its uniquely structured poultry industry.
在新西兰，2000 年至 2007 年间弯曲菌病的报告数量显著增加。值得注意的是，该国的家禽供应与许多发达国家不同，因为零售的新鲜和冷冻家禽仅来自国内。为了研究人类病例与家禽之间的可能联系，建立了一个监测哨点，以在 2005 年至 2008 年的 3 年期间使用多位点序列分型研究空肠弯曲菌的分子流行病学。研究表明，主要供应商提供的零售禽肉中，有 60.1%至 81.4%受到空肠弯曲菌的污染。个别家禽供应商和人类之间在污染的可能性和水平以及基因型的相对频率方面存在差异。一些禽肉受到属于多个序列型（ST）的分离株的污染，而且存在普遍存在和供应商相关的菌株的证据，这种流行模式在其他国家尚未得到认可。常见的禽肉 ST 也常见于人类临床病例中，这表明家禽是人类感染的主要来源。本研究发现了国际上罕见的基因型，如 ST-3069 和 ST-474，以及常见的基因型，如 ST-45 和 ST-48。在新西兰占主导地位的人类序列型 ST-474 几乎仅存在于一家家禽供应商的分离株中，这表明空肠弯曲菌在该国具有独特的分子流行病学特征。这些结果可能部分归因于新西兰的地理位置隔离及其独特的家禽产业结构。
