Jahanshahi Peyman, Zalnezhad Erfan, Sekaran Shamala Devi, Adikan Faisal Rafiq Mahamd
Photonics Research Group, Department of Electrical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Center of Advanced Manufacturing and Material Processing, Department of Engineering Design and Manufacture, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sci Rep. 2014 Jan 24;4:3851. doi: 10.1038/srep03851.
Surface plasmon resonance (SPR) is a medical diagnosis technique with high sensitivity and specificity. In this research, a new method based on SPR is proposed for rapid, 10-minute detection of the anti-dengue virus in human serum samples. This novel technique, known as rapid immunoglobulin M (IgM)-based dengue diagnostic test, can be utilized quickly and easily at the point of care. Four dengue virus serotypes were used as ligands on a biochip. According to the results, a serum volume of only 1 μl from a dengue patient (as a minimized volume) is required to indicate SPR angle variation to determine the ratio of each dengue serotype in samples with 83-93% sensitivity and 100% specificity.
表面等离子体共振（SPR）是一种具有高灵敏度和特异性的医学诊断技术。在本研究中，提出了一种基于SPR的新方法，用于在10分钟内快速检测人血清样本中的抗登革病毒。这种新技术，即基于快速免疫球蛋白M（IgM）的登革热诊断测试，可在护理点快速简便地使用。四种登革病毒血清型被用作生物芯片上的配体。根据结果，仅需1微升登革热患者的血清（作为最小化体积）即可指示SPR角度变化，以确定样本中每种登革热血清型的比例，灵敏度为83%-93%，特异性为100%。
