Institute of Dendrology, Polish Academy of Sciences, 5 Parkowa Str., 62-035 Kórnik, Poland.
Mycorrhiza. 2010 Oct;20(7):473-81. doi: 10.1007/s00572-010-0298-2. Epub 2010 Feb 13.
In this paper, we report the effect of Scots pine genotypes on ectomycorrhizal (ECM) community and growth, survival, and foliar nutrient composition of 2-year-old seedlings grown in forest bare-root nursery conditions in Lithuania. The Scots pine seeds originated from five stands from Latvia (P1), Lithuania (P2 and P3), Belarus (P4), and Poland (P5). Based on molecular identification, seven ECM fungal taxa were identified: Suillus luteus and Suillus variegatus (within the Suilloid type), Wilcoxina mikolae, Tuber sp., Thelephora terrestris, Cenococcum geophilum, and Russuloid type. The fungal species richness varied between five and seven morphotypes, depending on seed origin. The average species richness and relative abundance of most ECM morphotypes differed significantly depending on pine origin. The most essential finding of our study is the shift in dominance from an ascomycetous fungus like W. mikolae in P2 and P4 seedlings to basidiomycetous Suilloid species like S. luteus and S. variegatus in P1 and P5 seedlings. Significant differences between Scots pine origin were also found in seedling height, root dry weight, survival, and concentration of C, K, Ca, and Mg in the needles. The Spearman rank correlation coefficient revealed that survival and nutritional status of pine seedlings were positively correlated with abundance of Suilloid mycorrhizas and negatively linked with W. mikolae abundance. However, stepwise multiple regression analysis showed that only survival and magnesium content in pine needles were significantly correlated with abundance of ECM fungi, and Suilloid mycorrhizas were a main significant predictor. Our results may have implications for understanding the physiological and genetic relationship between the host tree and fungi and should be considered in management decisions in forestry and ECM fungus inoculation programs.
本文报道了来自拉脱维亚（P1）、立陶宛（P2 和 P3）、白俄罗斯（P4）和波兰（P5）五个林分的欧洲赤松基因型对菌根（ECM）群落以及在立陶宛森林裸根苗圃条件下生长的 2 年生幼苗的生长、存活和叶片养分组成的影响。基于分子鉴定，共鉴定出 7 种 ECM 真菌类群：S. luteus 和 S. variegatus（属于 Suilloid 型）、W. mikolae、Tuber sp.、T. terrestris、C. geophilum 和 Russuloid 型。真菌物种丰富度在 5 到 7 种形态型之间变化，这取决于种子的起源。来自不同松树起源的幼苗的 ECM 大多数形态型的物种丰富度和相对丰度存在显著差异。我们研究的最重要发现是，从 P2 和 P4 幼苗中占优势的子囊菌 W. mikolae 转变为 P1 和 P5 幼苗中占优势的担子菌 Suilloid 物种，如 S. luteus 和 S. variegatus。不同松树起源的幼苗在苗高、根干重、存活率以及针叶中 C、K、Ca 和 Mg 的浓度方面也存在显著差异。Spearman 秩相关系数表明，幼苗的存活率和营养状况与 Suilloid 菌根的丰度呈正相关，与 W. mikolae 的丰度呈负相关。然而，逐步多元回归分析表明，只有幼苗的存活率和针叶中的镁含量与 ECM 真菌的丰度显著相关，而 Suilloid 菌根是主要的显著预测因子。我们的研究结果可能对理解宿主树与真菌之间的生理和遗传关系具有重要意义，并且应该在林业管理决策和菌根真菌接种计划中考虑。
