Rincón Ana, de Felipe M R, Fernández-Pascual M
Departamento de Fisiología y Bioquímica Vegetal, Instituto de Recursos Naturales, Centro de Ciencias Medioambientales (IRN-CCMA-CSIC), C/ Serrano, 115 dupl., 28006, Madrid, Spain.
Mycorrhiza. 2007 Dec;18(1):23-32. doi: 10.1007/s00572-007-0149-y. Epub 2007 Sep 14.
Vegetative inoculum of Amanita ovoidea (Bull.) Link and three isolates of Suillus collinitus (Fr.) Kuntze, as well as spore inoculum of Rhizopogon roseolus (Corda) Th. M. Fr. and S. collinitus, were evaluated for the production of Pinus halepensis Mill. in nursery and for the establishment of seedlings in a degraded gypsum soil. In nursery, most of the fungi significantly improved the height of seedlings and modified the accumulation of nutrients in needles. The percentage of ectomycorrhizas (ESR) per seedling ranged from 25 to 78%, depending on the fungi. One and 2 years after planting in the field, the survival of seedlings was significantly improved by inoculation with two isolates of S. collinitus and with spores of the same fungus. Inoculation with A. ovoidea had no significant effect on seedling survival, whilst R. roseolus caused a significant mortality of seedlings. Seedling height was significantly improved by inoculation with all fungi except R. roseolus and isolate CCMA-1 of S. collinitus. One year after planting, mycorrhization of control seedlings was negligible, and percentages of ESR were under 38% for the rest of treatments. In spring of the second year, seedlings in all treatments, including the control, became highly mycorrhizal (60-77% of ESR). Low ectomycorrhizal diversity (five morphotypes described) and seasonal variation on morphotype composition were detected 2 years after plantation. From a perspective of soil restoration management under limiting environmental conditions, nursery inoculation with selected fungi can be a key advantage for tree seedlings to surmount the initial transplant stress, assuring their establishment in the field. Our results emphasise the importance of selecting compatible fungal-host species combinations for nursery inoculation and sources of inoculum adapted to the environmental conditions of the transplantation site.
对卵孢鹅膏菌（Amanita ovoidea (Bull.) Link）的营养接种体和三种黏盖牛肝菌（Suillus collinitus (Fr.) Kuntze）分离株，以及玫瑰红须腹菌（Rhizopogon roseolus (Corda) Th. M. Fr.）和黏盖牛肝菌的孢子接种体，进行了在苗圃中对阿勒颇松（Pinus halepensis Mill.）育苗以及在退化石膏土壤中使幼苗定植方面的评估。在苗圃中，大多数真菌显著提高了幼苗高度，并改变了针叶中养分的积累。每株幼苗的外生菌根（ESR）百分比在25%至78%之间，这取决于真菌种类。在田间种植1年和2年后，接种两种黏盖牛肝菌分离株以及同一真菌的孢子显著提高了幼苗的存活率。接种卵孢鹅膏菌对幼苗存活率没有显著影响，而玫瑰红须腹菌导致幼苗显著死亡。除玫瑰红须腹菌和黏盖牛肝菌分离株CCMA - 1外，接种所有真菌均显著提高了幼苗高度。种植1年后，对照幼苗的菌根化程度可忽略不计，其他处理的ESR百分比低于38%。在第二年春季，包括对照在内的所有处理中的幼苗都形成了高度菌根化（ESR为60 - 77%）。种植2年后检测到外生菌根多样性较低（描述了五种形态类型）且形态类型组成存在季节性变化。从在有限环境条件下土壤恢复管理的角度来看，在苗圃中接种选定真菌对于树苗克服初始移植应激、确保其在田间定植可能是一个关键优势。我们的结果强调了为苗圃接种选择相容的真菌 - 宿主物种组合以及适合移植地点环境条件的接种体来源的重要性。
