Institute of Food Safety and Environmental Monitoring, Fuzhou University, PR China.
Electrophoresis. 2010 Mar;31(6):1011-8. doi: 10.1002/elps.200900515.
A new method, pressurized CEC with end-column amperometric detection using carbon paste electrode, has been developed for the separation and determination of five phenolic xenoestrogens in chicken eggs and milk powder samples. Efficient separation of five analytes was performed by pressurized CEC using a mobile phase consisting of 60% v/v ACN and 40% v/v Tris buffer (5 mmol/L, pH 8.0), +6 kV of applied voltage and 7.0 MPa of supplementary pressure. Detection limits of 50, 5, 2, 10 and 20 ng/mL for pentachlorophenol, bisphenol-A, 2,4-dichlorophenol, 4-tert-octylphenol and 4-nonylphenol, respectively, were achieved using carbon paste electrode as working electrode and +0.8 V as detection potential. Matrix solid phase dispersion extraction method had been employed during sample preparation procedure, and mean recoveries ranged from 79.2 to 102.6% at different concentrations of phenolic xenoestrogens for spiked egg and milk powder samples were obtained.
一种新的方法，加压毛细管电泳与端柱安培检测使用碳糊电极，已经被开发出来用于分离和测定五种酚类环境雌激素在鸡蛋和奶粉样品。高效分离五种分析物采用加压毛细管电泳使用流动相由 60%的乙腈和 40%的 Tris 缓冲液（5mmol/L，pH 值 8.0），+6 kV 的外加电压和 7.0 MPa 的补充压力。检测限为 50、5、2、10 和 20ng/mL 五氯酚、双酚 A、2,4-二氯酚、4-叔辛基酚和 4-壬基酚，分别使用碳糊电极为工作电极和+0.8 V 为检测电位。在样品制备过程中采用了基质固相分散萃取法，在不同浓度的酚类环境雌激素下，对鸡蛋和奶粉样品进行了加标回收实验，回收率在 79.2%到 102.6%之间。
