Peng Jiangnan, Place Allen R, Yoshida Wesley, Anklin Clemens, Hamann Mark T
Department of Pharmacognosy and National Center for Natural Products Research, University of Mississippi, University, Mississippi 38677, USA.
J Am Chem Soc. 2010 Mar 17;132(10):3277-9. doi: 10.1021/ja9091853.
In an attempt to determine the cause of repeated fish kills in an estuarine aquaculture facility in Maryland, a toxin with hemolytic, cytotoxic, and ichthyotoxic properties, designated as karlotoxin-2 (KmTx2), was isolated from Karlodinium veneficum. The structure of KmTx2 was elucidated by means of detailed ID and 2D NMR spectra, including 2D INADEQUATE. The relative and absolute configurations of KmTx2 were determined using J-based configuration analysis and comparison of its degradation products with synthetic controls.
为了确定马里兰州一个河口水产养殖设施中鱼类反复死亡的原因，从剧毒卡尔藻（Karlodinium veneficum）中分离出了一种具有溶血、细胞毒性和鱼毒性的毒素，命名为卡罗毒素-2（KmTx2）。通过详细的一维和二维核磁共振谱（包括二维全相关谱）阐明了KmTx2的结构。利用基于耦合常数的构型分析以及将其降解产物与合成对照品进行比较，确定了KmTx2的相对构型和绝对构型。
