从东海的卡尔多利毒素（GM2 株）中发现两种新的卡尔多毒素。
Two new karlotoxins found in Karlodinium veneficum (strain GM2) from the East China Sea.
机构信息
LiDakSum Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China; Center for Collaborative Innovation, Ningbo University, Ningbo 315211, China.
LiDakSum Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China; Center for Collaborative Innovation, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education, Ningbo 315211, China.
出版信息
Harmful Algae. 2016 Sep;58:66-73. doi: 10.1016/j.hal.2016.08.001. Epub 2016 Aug 27.
Abstract
The dinoflagellate Karlodinium veneficum is a harmful algal bloom species with a worldwide distribution. This small athecate dinoflagellate makes a family of polyketide toxins that are hemolytic, cytotoxic and ichthyotoxic. The first chemical structure for karlotoxins from East China Sea (ECS) is reported here. The two new karlotoxins, namely 4,5-dihydro-KmTx 2 (compound 1) and 4,5-dihydro-dechloro-KmTx 2 (compound 2), were isolated and purified from monoalgal cultures of K. veneficum strain GM2. Their structures were determined by spectroscopic analysis, including tandem mass spectrometry as well as 1D and 2D NMR experiments. These new karlotoxin congeners feature a saturated polyol arm different from previously reported for KmTx 2 that appears to increase hemolytic activity.
摘要
卡盾藻属的卡尔多林是一种具有全球分布的有害赤潮物种。这种小型无壁甲藻产生一组聚酮类毒素，具有溶血、细胞毒性和鱼毒性。本文首次报道了东海（ECS）卡多毒素的化学结构。从卡盾藻 GM2 单藻培养物中分离和纯化了两种新的卡多毒素，即 4,5-二氢-KmTx2（化合物 1）和 4,5-二氢-去氯-KmTx2（化合物 2）。它们的结构通过光谱分析确定，包括串联质谱以及 1D 和 2D NMR 实验。这些新的卡多毒素同系物具有不同于以前报道的 KmTx2 的饱和多醇臂，似乎增加了溶血活性。
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