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基于微乳液的有机凝胶作为脂肪酶固定化的基质。

Microemulsion-based organogels as matrices for lipase immobilization.

机构信息

Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave 11635, Athens, Greece.

出版信息

Biotechnol Adv. 2010 May-Jun;28(3):395-406. doi: 10.1016/j.biotechadv.2010.02.004. Epub 2010 Feb 13.

Abstract

Organogels based on water-in-oil microemulsions can be formed using various natural polymers such as gelatin, agar or cellulose derivatives. Enzymes entrapped in the water core of the microemulsion can keep their activity and enhance their stability within the gel matrix. The importance of the microemulsion based organogels (MBGs) leans on their numerous potential biotechnological applications. An important example is the use of various lipase microemulsion systems for hydrolytic or synthetic reactions. In this review, several MBGs are being evaluated as immobilization matrices for various enzymes. The main subject focuses on the parameters that affect the use of MBGs as media for bioorganic reactions using lipases as catalysts.

摘要

基于油包水乳状液的有机凝胶可以使用各种天然聚合物形成,如明胶、琼脂或纤维素衍生物。包埋在乳状液水核中的酶可以保持其活性,并在凝胶基质中提高其稳定性。基于微乳液的有机凝胶(MBGs)的重要性在于它们具有许多潜在的生物技术应用。一个重要的例子是使用各种脂肪酶微乳液系统进行水解或合成反应。在这篇综述中,几种 MBGs 被评估为各种酶的固定化基质。主要的主题集中在影响使用 MBGs 作为生物有机反应介质的参数上,其中脂肪酶作为催化剂。

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