Vassiliadi Evdokia, Mitsou Evgenia, Avramiotis Spyridon, Chochos Christos L, Pirolt Franz, Medebach Martin, Glatter Otto, Xenakis Aristotelis, Zoumpanioti Maria
Institute of Chemical Biology, National Hellenic Research Foundation, 48, Vassileos Constantinou Ave., 11635 Athens, Greece.
Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece.
Nanomaterials (Basel). 2020 Nov 5;10(11):2204. doi: 10.3390/nano10112204.
(Hydroxypropyl)methyl cellulose (HPMC) can be used to form gels integrating a w/o microemulsion. The formulation in which a microemulsion is mixed with a hydrated HPMC matrix has been successfully used as a carrier of biocompatible ingredients. However, little is known about the structure of these systems. To elucidate this, scanning electron microscopy was used to examine the morphology and the bulk of the microemulsion-based gels (MBGs) and small-angle X-ray scattering to clarify the structure and detect any residual reverse micelles after microemulsion incorporation in the gel. Electron paramagnetic resonance spectroscopy was applied using spin probes to investigate the polar and non-polar areas of the gel. Furthermore, the enzyme-labelling technique was followed to investigate the location of an enzyme in the matrix. A structural model for HPMC matrix is proposed according to which, although a w/o microemulsion is essential to form the final gel, no microemulsion droplets can be detected after incorporation in the gel. Channels are formed by the organic solvent (oil), which are coated by surfactant molecules and a water layer in which the enzyme can be hosted.
羟丙基甲基纤维素（HPMC）可用于形成包含水包油型微乳液的凝胶。微乳液与水合HPMC基质混合的配方已成功用作生物相容性成分的载体。然而，对于这些体系的结构了解甚少。为阐明这一点，使用扫描电子显微镜检查基于微乳液的凝胶（MBG）的形态和整体结构，并使用小角X射线散射来阐明结构并检测微乳液掺入凝胶后是否存在残留的反胶束。使用自旋探针应用电子顺磁共振光谱来研究凝胶的极性和非极性区域。此外，采用酶标记技术来研究酶在基质中的位置。提出了一种HPMC基质的结构模型，根据该模型，尽管水包油型微乳液对于形成最终凝胶至关重要，但在掺入凝胶后无法检测到微乳液滴。通道由有机溶剂（油）形成，这些通道由表面活性剂分子和可容纳酶的水层包覆。
