Division of Biological Sciences and The Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Korea.
Immune Netw. 2009 Oct;9(5):203-7. doi: 10.4110/in.2009.9.5.203. Epub 2009 Oct 30.
The FimA of Porphyromonas gingivalis is a crucial pathogenic component of the bacteria and has been implicated as a target for vaccine development against the periodontal diseases.
In this study, the purified fimbriae (FimA subunit polymers) protein was used for immunization in their native form and B hybridoma clones producing antibodies specific to FimA were established.
The monoclonal antibodies prepared from selected two clones, designated #123 (IgG2b/ kappa) and #265 (IgG1/kappa), displayed different patterns of binding activity against the cognate antigen. Both antibodies reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the monoclonal antibodies showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface.
These results suggest that the monoclonal antibodies could be used as vaccine material against the periodontal diseases through passive immunization.
牙龈卟啉单胞菌的 FimA 是该细菌的关键致病成分，已被认为是针对牙周病的疫苗开发的目标。
在这项研究中，使用纯化的菌毛（FimA 亚基聚合物）蛋白以其天然形式进行免疫接种，并建立了产生针对 FimA 的特异性抗体的 B 杂交瘤克隆。
从选定的两个克隆中制备的单克隆抗体，分别命名为 #123（IgG2b/κ）和 #265（IgG1/κ），显示出针对同源抗原的不同结合活性模式。两种抗体均与部分解离的低聚物表达的构象表位反应，但如 Western blot 分析所示，不与单体反应。含有单克隆抗体的腹水液显示出对唾液包被羟基磷灰石珠的抑制活性，这是一种用于牙表面涂层的薄膜包被的体外模型。
这些结果表明，通过被动免疫，这些单克隆抗体可用作针对牙周病的疫苗材料。
