Department of Molecular Biology, Chonbuk National University, Jeonju, Korea.
Microbiol Immunol. 2011 Mar;55(3):199-210. doi: 10.1111/j.1348-0421.2011.00305.x.
FimA of Porphyromonas gingivalis, a major pathogen in periodontitis, is known to be closely related to the virulence of these bacteria and has been suggested as a candidate for development of a vaccine against periodontal disease. In order to develop a passive immunization method for inhibiting the establishment of periodontal disease, B hybridoma clones 123-123-10 and 256-265-9, which produce monoclonal antibodies (Mabs) specific to purified fimbriae, were established. Both mAbs reacted with the conformational epitopes displayed by partially dissociated oligomers of FimA, but not with the 43 kDa FimA monomer. Gene sequence analyses of full-length cDNAs encoding heavy and light chain immunoglobulins enabled classification of the genes of mAb 123-123-10 as members of the mVh II (A) and mVκ I subgroups, and those of mAb 256-265-9 as members of the mVh III (D) and mVκ I subgroups. More importantly, 50 ng/mL of antibodies purified from the culture supernatant of antibody gene-transfected CHO cells inhibited, by approximately 50%, binding of P. gingivalis to saliva-coated hydroxyapatite bead surfaces. It is expected that these mAbs could be used as a basis for passive immunization against P. gingivalis-mediated periodontitis.
牙龈卟啉单胞菌（Porphyromonas gingivalis）的 FimA 是牙周炎的主要病原体之一，与这些细菌的毒力密切相关，被认为是牙周病疫苗开发的候选物。为了开发抑制牙周病发生的被动免疫方法，我们建立了产生针对纯化菌毛的单克隆抗体（mAb）的 B 杂交瘤克隆 123-123-10 和 256-265-9。两种 mAb 均与 FimA 部分解离寡聚体显示的构象表位反应，但不与 43 kDa 的 FimA 单体反应。全长 cDNA 编码重链和轻链免疫球蛋白的基因序列分析将 mAb 123-123-10 的基因归类为 mVh II（A）和 mVκ I 亚群，而 mAb 256-265-9 的基因归类为 mVh III（D）和 mVκ I 亚群。更重要的是，从转抗体基因 CHO 细胞的培养上清液中纯化的 50ng/ml 抗体可抑制约 50%的 P. gingivalis 与唾液包被的羟磷灰石珠表面的结合。预计这些 mAb 可用作针对牙龈卟啉单胞菌介导的牙周炎的被动免疫的基础。
