Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 Project, Seoul, Republic of Korea.
J Mater Sci Mater Med. 2012 Mar;23(3):753-62. doi: 10.1007/s10856-011-4473-6. Epub 2011 Nov 10.
In vitro cytotoxicity test is an initial step to identify the harmful effects of new dental materials. Aim of this study was to develop a stable human cell line derived from normal gingival fibroblasts (hNOF) and to assess its feasibility in in vitro cytotoxicity testing. Immortalized human gingival fibroblasts (hTERT-hNOF) were successfully established with human telomerase reverse transcriptase gene transfection, preserving its phenotypical characteristics, replicative potential and biological properties. Utilizing standard cytotoxicity test modeling and dental materials, hTERT-hNOF were evaluated for their feasibility in cytotoxicity testing, compared with hNOF and L929 cells. Similar pattern of cytotoxic response was observed among hNOF, hTERT-hNOF and L929 cells. Cytotoxicity response of hTERT-hNOF was significantly similar to hNOF, moreover hTERT-hNOF and hNOF were found to be more sensitive towards the tested dental materials compared to L929 cells. This study suggested that hTERT-hNOF is an effective cytotoxic test model for dental materials.
体外细胞毒性试验是识别新型牙科材料有害作用的初始步骤。本研究旨在建立一种源自正常牙龈成纤维细胞（hNOF）的稳定人细胞系，并评估其在体外细胞毒性试验中的可行性。成功地通过人端粒酶逆转录酶基因转染建立了永生化的人牙龈成纤维细胞（hTERT-hNOF），保留了其表型特征、复制潜能和生物学特性。利用标准细胞毒性试验模型和牙科材料，评估 hTERT-hNOF 在细胞毒性试验中的可行性，并与 hNOF 和 L929 细胞进行比较。在 hNOF、hTERT-hNOF 和 L929 细胞中观察到相似的细胞毒性反应模式。hTERT-hNOF 的细胞毒性反应与 hNOF 非常相似，此外，与 L929 细胞相比，hTERT-hNOF 和 hNOF 对测试的牙科材料更为敏感。本研究表明，hTERT-hNOF 是一种有效的牙科材料细胞毒性试验模型。
