Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul 100-715, South Korea.
J Biosci Bioeng. 2010 Mar;109(3):291-6. doi: 10.1016/j.jbiosc.2009.08.478. Epub 2009 Sep 18.
Labisia pumila (Myrsinaceae), known as "Kacip Fatimah," has been used by many generations of Malay women to induce and facilitate child birth as well as a post partum medicine. However, its topical application on skin has not been reported yet. In this study, we have focused on the anti-photoaging effects of L. pumila. Extract of L. pumila was first analyzed for their antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl) since UV irradiation is a primary cause of reactive oxygen species (ROS) generation in the skin. The 50% free radical scavenging activity (FSC(50)) of L. pumila extract was determined to be 0.006%, which was equal to that produced by 156 microM ascorbic acid. TNF-alpha and cyclooxygenase (COX-2) play a primary role in the inflammation process upon UV irradiation and are known to be stimulated by UVB. Treatment with L. pumila extract markedly inhibited the TNF-alpha production and the expression of COX-2. Decreased collagen synthesis of human fibroblasts by UVB was restored back to normal level after treatment with L. pumila extract. On the other hand, the enhanced MMP-1 expression upon UVB irradiation was down regulated by L. pumila extract in a dose-dependent manner. Furthermore, treatment of normal keratinocytes with L. pumila extract attenuated UVB-induced MMP-9 expression. These results collectively suggest L. pumila extract has tremendous potential as an anti-photoaging cosmetic ingredient.
鸡矢藤（茜草科），俗称“子母草”，被许多代马来妇女用来引产和促进分娩，以及产后药物。然而，它在皮肤上的局部应用尚未有报道。在这项研究中，我们专注于鸡矢藤的抗光老化作用。鸡矢藤提取物首先用 DPPH（2,2-二苯基-1-苦基肼）分析其抗氧化活性，因为紫外线照射是皮肤中活性氧（ROS）产生的主要原因。鸡矢藤提取物的 50％自由基清除活性（FSC（50））为 0.006％，与 156μM 抗坏血酸相当。TNF-α和环氧化酶（COX-2）在 UV 照射后的炎症过程中起主要作用，并且已知它们受 UVB 刺激。鸡矢藤提取物处理显著抑制 TNF-α的产生和 COX-2 的表达。鸡矢藤提取物处理可将 UVB 诱导的人成纤维细胞胶原合成减少恢复至正常水平。另一方面，鸡矢藤提取物以剂量依赖的方式下调 UVB 照射诱导的 MMP-1 表达。此外，鸡矢藤提取物处理正常角质形成细胞可减轻 UVB 诱导的 MMP-9 表达。这些结果共同表明鸡矢藤提取物具有作为抗光老化化妆品成分的巨大潜力。
